Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
Key features and details
- Mouse monoclonal [RKSE60] to Cytokeratin 10
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Pig, Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 10 antibody [RKSE60]
See all Cytokeratin 10 primary antibodies -
Description
Mouse monoclonal [RKSE60] to Cytokeratin 10 -
Host species
Mouse -
Specificity
This antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas -
Tested applications
Suitable for: IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Pig, Zebrafish -
Immunogen
Full length native protein (purified) corresponding to Human Cytokeratin 10. Cytokeratins from the human epidermis.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RKSE60 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Tissue specificity
Seen in all suprabasal cell layers including stratum corneum. -
Involvement in disease
Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Alternative names
- BCIE antibody
- BIE antibody
- CK 10 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab9025 has been referenced in 24 publications.
- Tsujikawa K et al. Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head. Dev Dyn 251:1107-1122 (2022). PubMed: 34997665
- Erratico S et al. Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healing. Cell Mol Life Sci 79:259 (2022). PubMed: 35474498
- Polkoff KM et al. LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis. Sci Rep 12:9104 (2022). PubMed: 35650234
- Liu LP et al. Transcriptomic and Functional Evidence Show Similarities between Human Amniotic Epithelial Stem Cells and Keratinocytes. Cells 11:N/A (2021). PubMed: 35011631
- Ruiz-Torres S et al. Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin. Cell Stem Cell 28:424-435.e6 (2021). PubMed: 33232662