Anti-Cytokeratin 16/K16 antibody (ab182791)
Key features and details
- Rabbit polyclonal to Cytokeratin 16/K16
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 16/K16 antibody
See all Cytokeratin 16/K16 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 16/K16 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Cytokeratin 16/K16 aa 150-250. Conjugated to keyhole limpet hemocyanin.
Database link: P08779 -
Positive control
- Human cervical cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 51 kDa.
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Notes |
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IHC-P
1/25 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 51 kDa. |
Target
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Tissue specificity
Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts. -
Involvement in disease
Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3868 Human
- Entrez Gene: 16666 Mouse
- Omim: 148067 Human
- SwissProt: P08779 Human
- SwissProt: Q9Z2K1 Mouse
- Unigene: 655160 Human
- Unigene: 422799 Mouse
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Alternative names
- CK 16 antibody
- CK-16 antibody
- CK16 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human cervical cancer tissue labeling Cytokeratin 16/K16 with ab182791 at 1/25. Image on the right is treated with the synthetic peptide.
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Anti-Cytokeratin 16/K16 antibody (ab182791) at 1/500 dilution + Human liver tissue at 30 µg
Secondary
Goat anti Rabbit IgG at 1/5000 dilution
Predicted band size: 51 kDa
Exposure time: 1 minute
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab182791 has not yet been referenced specifically in any publications.