Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Other biological fluids, Suspension cells, Tissue
Product nameD-Gluconate Assay Kit (Colorimetric)
Sample typeOther biological fluids, Tissue, Adherent cells, Suspension cells
D-Gluconate Assay Kit (Colorimetric) (ab204703) is a sensitive, fast and easy-to-use kit. In this assay, Gluconate is utilized by Gluconokinase to form D-Gluconate-6-P and ADP, which subsequently undergoes a series of reactions to form an intermediate that reduces Gluconate probe to give a product with strong absorbance at 450 nm. This assay kit can detect D-Gluconate (D-Gluconic Acid) level less than 2 µM in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K683 D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric). K683-100 is the same size as the 100 test size of ab204703.
D-Gluconic acid (C6H12O7) is a mild organic acid that is produced from glucose by glucose oxidase. It is abundantly present in plants, fruits and animal tissues. D-Gluconate (C6H11O7) is the salt or ester of Gluconic Acid. Due to its low toxicity, it is widely used in pharmaceutical, food, and other industries.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests ATP 1 vial Gluconate Assay Buffer 1 x 25ml Gluconate Converter 1 vial Gluconate Developer 1 vial Gluconate Enzyme Mix 1 vial Gluconate Probe 1 vial Gluconate Standard 1 vial
ab204703 has not yet been referenced specifically in any publications.