Anti-DAD1 antibody (ab23836)
Key features and details
- Rabbit polyclonal to DAD1
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DAD1 antibody -
Description
Rabbit polyclonal to DAD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).
-
Positive control
- HepG2 cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified by affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
|
ICC |
Use at an assay dependent dilution.
|
Notes |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa). |
ICC
Use at an assay dependent dilution. |
Target
-
Function
Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. -
Sequence similarities
Belongs to the DAD/OST2 family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1603 Human
- GenBank: NP_001335.1 Human
- Omim: 600243 Human
- SwissProt: P61803 Human
- Unigene: 82890 Human
-
Alternative names
- DAD 1 antibody
- DAD-1 antibody
- dad1 antibody
see all
Images
-
Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml
All lanes : HepG2 whole cell lysate
Predicted band size: 12 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab23836 has been referenced in 2 publications.
- Ding Y et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . PubMed: 25301944
- Murooka TT et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . PubMed: 19607806