Anti-DAK antibody (ab137623)
Key features and details
- Rabbit polyclonal to DAK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DAK antibody -
Description
Rabbit polyclonal to DAK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 273-548 of Human DAK
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Positive control
- HepG2 and Raji whole cell lysates, Human lung SCC, HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes both the phosphorylation of dihydroxyacetone and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. -
Sequence similarities
Belongs to the dihydroxyacetone kinase (DAK) family.
Contains 1 DhaK domain.
Contains 1 DhaL domain. - Information by UniProt
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Database links
- Entrez Gene: 512373 Cow
- Entrez Gene: 26007 Human
- SwissProt: Q58DK4 Cow
- SwissProt: Q3LXA3 Human
- Unigene: 6278 Human
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Alternative names
- ATP dependent dihydroxyacetone kinase antibody
- Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing) antibody
- DAK antibody
see all
Images
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All lanes : Anti-DAK antibody (ab137623) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human lung SCC labelling DAK with ab137623 antibody at a dilution of 1/100.
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling DAK with ab137623 at 1/100 dilution. Picture at the bottom costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137623 has not yet been referenced specifically in any publications.