Product nameAnti-DARC antibody
See all DARC primary antibodies
DescriptionRabbit polyclonal to DARC
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
A 16 amino acid peptide from near the carboxy terminus of human DARC.
- WB: Mouse brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab58965is purified.
Our Abpromise guarantee covers the use of ab58965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
FunctionNon-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
Tissue specificityFound in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
Sequence similaritiesBelongs to the G-protein coupled receptor Duffy family.
- Information by UniProt
- ACKR1 antibody
- Atypical chemokine receptor 1 antibody
- CCBP1 antibody
Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/ml
Lane 2 : Anti-DARC antibody (ab58965) at 1 µg/ml
Lane 3 : Anti-DARC antibody (ab58965) at 2 µg/ml
All lanes : mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Lanes 1-2 : Anti-rabbit IgG HRP at 1/1000 dilution
Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Samaniego R et al. Mesenchymal contribution to recruitment, infiltration, and positioning of leukocytes in human melanoma tissues. J Invest Dermatol 133:2255-64 (2013). Human . Read more (PubMed: 23446986) »
- Wessels JM et al. Expression of chemokine decoy receptors and their ligands at the porcine maternal-fetal interface. Immunol Cell Biol : (2010). Pig . Read more (PubMed: 20680026) »