Anti-DBC1 antibody - N-terminal (ab183850)
Key features and details
- Rabbit polyclonal to DBC1 - N-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-DBC1 antibody - N-terminal
See all DBC1 primary antibodies -
Description
Rabbit polyclonal to DBC1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human DBC1 aa 1-250 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60477 -
Positive control
- MCF7 whole cell lysate; MCF7 cells; mouse kidney and rat hind brain tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
|
IHC-P |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
-
Function
Inhibits cell proliferation by negative regulation of the G1/S transition. Mediates cell death which is not of the classical apoptotic type and regulates expression of components of the plasminogen pathway. -
Tissue specificity
Highly expressed in brain. Weakly expressed in heart, lung, skeletal muscle, kidney, thymus, prostate, testis and small intestine. -
Sequence similarities
Belongs to the FAM5 family.
Contains 1 MACPF domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 1620 Human
- Entrez Gene: 56710 Mouse
- Entrez Gene: 140610 Rat
- Omim: 602865 Human
- SwissProt: O60477 Human
- SwissProt: Q920P3 Mouse
- SwissProt: Q925T8 Rat
- Unigene: 532316 Human
-
Alternative names
- BMP/retinoic acid-inducible neural-specific protein 1 antibody
- BRINP1 antibody
- DBC1 antibody
see all
Images
-
Anti-DBC1 antibody - N-terminal (ab183850) at 1/500 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 89 kDa -
Immunohistochemical analysis of paraffin embedded rat hind brain tissue labeling DBC1 with ab183850 at 1/500.
-
Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling DBC1 with ab183850 at 1/500.
-
Immunofluorescent analysis of MCF7 cells labeling DBC1 with ab183850 at 1/500. Image at the right stained with Hoechst 33342. Note: cells were fixed in 100% MeOH for 5 min.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab183850 has not yet been referenced specifically in any publications.