- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-DDAH2 antibody
See all DDAH2 primary antibodies -
DescriptionRabbit polyclonal to DDAH2
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog
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Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GTPGEGLGRC SHALIRGVPE SLASGEGAGA GLPALDLAKA QREHGVLGGK) of Human DDAH2 (NP_039268).
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Positive control
- Human fetal brain lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab87064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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FunctionHydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
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Tissue specificityDetected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
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Sequence similaritiesBelongs to the DDAH family.
- Information by UniProt
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Database links
- Entrez Gene: 540386 Cow
- Entrez Gene: 23564 Human
- Entrez Gene: 51793 Mouse
- Entrez Gene: 294239 Rat
- Omim: 604744 Human
- SwissProt: Q3SX44 Cow
- SwissProt: O95865 Human
- SwissProt: Q99LD8 Mouse
see all -
Alternative names
- DDAH antibody
- DDAH II antibody
- DDAH-2 antibody
see all
Images
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Western blot - Anti-DDAH2 antibody (ab87064)Anti-DDAH2 antibody (ab87064) at 1 µg/ml (5% skim milk / PBS buffer ) + Human fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Gel concentration: 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab87064)ab87064 at 5 µg/ml staining DDAH2 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH2 antibody (ab87064)ab87064 at 5 µg/ml staining DDAH2 in Human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References
ab87064 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"