Anti-DDX19B antibody (ab70305)
Key features and details
- Rabbit polyclonal to DDX19B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DDX19B antibody
See all DDX19B primary antibodies -
Description
Rabbit polyclonal to DDX19B -
Host species
Rabbit -
Specificity
The immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human DDX19B. (NP_009173.1.)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
ATP-dependent RNA helicase involved in mRNA export from the nucleus. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils. - Information by UniProt
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Database links
- Entrez Gene: 11269 Human
- Entrez Gene: 234733 Mouse
- Omim: 605812 Human
- SwissProt: Q9UMR2 Human
- Unigene: 221761 Human
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Alternative names
- ATP-dependent RNA helicase DDX19B antibody
- DBP5 antibody
- Dbp5, yeast, homolog of antibody
see all
Images
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All lanes : Anti-DDX19B antibody (ab70305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70305 has been referenced in 1 publication.
- Park Y et al. Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF-DDX19B interaction. Nucleic Acids Res 49:8261-8276 (2021). PubMed: 34232997