Anti-DDX4 / MVH antibody (ab13840)
Key features and details
- Rabbit polyclonal to DDX4 / MVH
- Suitable for: WB, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-DDX4 / MVH antibody
See all DDX4 / MVH primary antibodies -
Description
Rabbit polyclonal to DDX4 / MVH -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Pig, Platypus
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Immunogen
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Positive control
- ICC: mES cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841).
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| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| IHC-P | (11) |
Use at an assay dependent concentration.
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| IHC-Fr | (7) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841). |
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. -
Tissue specificity
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. - Information by UniProt
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Database links
- Entrez Gene: 493725 Cow
- Entrez Gene: 54514 Human
- Entrez Gene: 13206 Mouse
- Entrez Gene: 310090 Rat
- Omim: 605281 Human
- SwissProt: Q5W5U4 Cow
- SwissProt: Q9NQI0 Human
- SwissProt: Q61496 Mouse
see all -
Alternative names
- DDX 4 antibody
- Ddx4 antibody
- DDX4_HUMAN antibody
see all
Images
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Immunocytochemistry - Anti-DDX4 / MVH antibody (ab13840)
ab13840 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab13840 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of Petra Hajkova, Gurdon Institute, University of CambridgeMouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1
µ The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
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Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 28 kDa, 47 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : ab13840 at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 52 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse Ovary Lysate at 10 µg
Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
Lane 3 : Mouse Ovary Lysate at 10 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Predicted band size: 76 kDa
Observed band size: 76 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (409)
ab13840 has been referenced in 409 publications.
- Miura K et al. Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3†. Biol Reprod 104:223-233 (2021). PubMed: 32965494
- Kinoshita M et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell 28:453-471.e8 (2021). PubMed: 33271069
- Subramanian GN et al. Premature ovarian ageing following heterozygous loss of Senataxin. Mol Hum Reprod 27:N/A (2021). PubMed: 33337500
- Liu X et al. The N-terminal BRCT domain determines MCPH1 function in brain development and fertility. Cell Death Dis 12:143 (2021). PubMed: 33542216
- Feng CW et al. Identification of regulatory elements required for Stra8 expression in fetal ovarian germ cells of the mouse. Development 148:N/A (2021). PubMed: 33574039