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    ddx4--mvh-antibody-ab13840.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families Other
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Anti-DDX4 / MVH antibody (ab13840)

  • Datasheet
  • SDS
Reviews (22)Q&A (24)References (412)

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Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody (ab13840)
  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)
  • Western blot - Anti-DDX4 / MVH antibody (ab13840)
  • Western blot - Anti-DDX4 / MVH antibody (ab13840)
  • Western blot - Anti-DDX4 / MVH antibody (ab13840)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)

Key features and details

  • Rabbit polyclonal to DDX4 / MVH
  • Suitable for: WB, ICC/IF, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-DDX4 / MVH antibody
    See all DDX4 / MVH primary antibodies
  • Description

    Rabbit polyclonal to DDX4 / MVH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Cow, Pig, Platypus
  • Immunogen

    Synthetic peptide within Human DDX4/ MVH aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9NQI0
    (Peptide available as ab13841)

  • Positive control

    • ICC: mES cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • DDX4 / MVH peptide (ab13841)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human DDX4 / MVH protein (ab153378)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841).
ICC/IF (2)
Use a concentration of 1 µg/ml.
IHC-P (11)
Use at an assay dependent concentration.
IHC-Fr (7)
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Function

    Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
  • Tissue specificity

    Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
  • Target information above from: UniProt accession Q9NQI0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 493725 Cow
    • Entrez Gene: 54514 Human
    • Entrez Gene: 13206 Mouse
    • Entrez Gene: 310090 Rat
    • Omim: 605281 Human
    • SwissProt: Q5W5U4 Cow
    • SwissProt: Q9NQI0 Human
    • SwissProt: Q61496 Mouse
    • SwissProt: Q6GWX0 Pig
    • SwissProt: Q64060 Rat
    • Unigene: 223581 Human
    • Unigene: 12818 Mouse
    • Unigene: 198577 Rat
    see all
  • Alternative names

    • DDX 4 antibody
    • Ddx4 antibody
    • DDX4_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
    • DEAD box protein 4 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
    • MVH antibody
    • Probable ATP dependent RNA helicase DDX4 antibody
    • Probable ATP-dependent RNA helicase DDX4 antibody
    • VASA antibody
    • Vasa homolog antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody (ab13840)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody (ab13840)

    ab13840 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab13840 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)
    Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of Petra Hajkova, Gurdon Institute, University of Cambridge

    Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1 µg/ml) overnight at 4 degrees. ab13840 staining is shown in red and Oct4 staining is shown in green. 

    The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.

     

     

     

     

  • Western blot - Anti-DDX4 / MVH antibody (ab13840)
    Western blot - Anti-DDX4 / MVH antibody (ab13840)
    All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml

    Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 76 kDa
    Additional bands at: 28 kDa, 47 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-DDX4 / MVH antibody (ab13840)
    Western blot - Anti-DDX4 / MVH antibody (ab13840)
    All lanes : ab13840 at 1 µg/ml

    Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 2 : Human testis tissue lysate - total protein

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 76 kDa
    Observed band size: 79 kDa why is the actual band size different from the predicted?
    Additional bands at: 47 kDa, 52 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-DDX4 / MVH antibody (ab13840)
    Western blot - Anti-DDX4 / MVH antibody (ab13840)
    All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml

    Lane 1 : Mouse Ovary Lysate at 10 µg
    Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
    Lane 3 : Mouse Ovary Lysate at 10 µg with DDX4 / MVH peptide (ab13841) at 1 µg/ml
    Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg with DDX4 / MVH peptide (ab13841) at 1 µg/ml

    Predicted band size: 76 kDa
    Observed band size: 76 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)
    IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (412)

Publishing research using ab13840? Please let us know so that we can cite the reference in this datasheet.

ab13840 has been referenced in 412 publications.

  • Miura K  et al. Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3†. Biol Reprod 104:223-233 (2021). PubMed: 32965494
  • Kinoshita M  et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell 28:453-471.e8 (2021). PubMed: 33271069
  • Subramanian GN  et al. Premature ovarian ageing following heterozygous loss of Senataxin. Mol Hum Reprod 27:N/A (2021). PubMed: 33337500
  • Liu X  et al. The N-terminal BRCT domain determines MCPH1 function in brain development and fertility. Cell Death Dis 12:143 (2021). PubMed: 33542216
  • Feng CW  et al. Identification of regulatory elements required for Stra8 expression in fetal ovarian germ cells of the mouse. Development 148:N/A (2021). PubMed: 33574039
View all Publications for this product

Customer reviews and Q&As

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Submit a review Submit a question

1-10 of 46 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (E13.5 Saggital gonad sections)
Permeabilization
Yes - Triton
Specification
E13.5 Saggital gonad sections
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

DR. Alain Chedotal

Verified customer

Submitted Aug 09 2021

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Sagittal embryonic mouse sections (E12.5), gonad)
Permeabilization
Yes - Triton
Specification
Sagittal embryonic mouse sections (E12.5), gonad
Blocking step
Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25%
Fixative
Paraformaldehyde
Read More

DR. Alain Chedotal

Verified customer

Submitted Aug 09 2021

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryo testis)
Permeabilization
Yes - Triton
Specification
Human embryo testis
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

DR. Alain Chedotal

Verified customer

Submitted Mar 03 2021

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (germ cells)
Permeabilization
No
Specification
germ cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 12 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-DDX4 / MVH antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Germline stem cell)
Permeabilization
Yes - 1% Triton X in PBS
Specification
Germline stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 20 2018

Western blot abreview for Anti-DDX4 / MVH antibody - Primordial Germ Cell Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Ovary)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Specification
Ovary
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Abcam user community

Verified customer

Submitted Jul 21 2016

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody - Primordial Germ Cell Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Ovary)
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formalin
Read More

Abcam user community

Verified customer

Submitted Jun 28 2016

Immunohistochemistry (Frozen sections) abreview for Anti-DDX4 / MVH antibody - Primordial Germ Cell Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

MR. Bryan Niedenberger

Verified customer

Submitted Mar 04 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DDX4 / MVH antibody - Primordial Germ Cell Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
Sample
Sheep Tissue sections (1-2 month old testis)
Specification
1-2 month old testis
Permeabilization
No
Fixative
4%PFA
Read More

Abcam user community

Verified customer

Submitted Jan 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DDX4 / MVH antibody - Primordial Germ Cell Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
Sample
Mouse Tissue sections (adult ovary)
Specification
adult ovary
Permeabilization
No
Fixative
4%PFA
Read More

Dr. Qing Wen

Verified customer

Submitted Apr 23 2014

1-10 of 46 Abreviews or Q&A

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