Anti-DDX4 / MVH antibody (ab13840)
- Datasheet
- References (304)
- Protocols
Overview
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Product name
Anti-DDX4 / MVH antibody
See all DDX4 / MVH primary antibodies -
Description
Rabbit polyclonal to DDX4 / MVH -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Cow, Human, Pig, Platypus -
Immunogen
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Positive control
- This antibody gave a positive signal in Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate and Human Testis Tissue Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab13840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841). | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 23598447 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/200. PubMed: 19440488 | |
| IHC-Fr | Use a concentration of 1 µg/ml. | |
| ICC/IF | 1/100. | |
| IHC-FoFr | 1/400. |
Target
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Function
Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. -
Tissue specificity
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. - Information by UniProt
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Database links
- Entrez Gene: 493725 Cow
- Entrez Gene: 54514 Human
- Entrez Gene: 13206 Mouse
- Entrez Gene: 310090 Rat
- Omim: 605281 Human
- SwissProt: Q5W5U4 Cow
- SwissProt: Q9NQI0 Human
- SwissProt: Q61496 Mouse
see all -
Alternative names
- DDX 4 antibody
- Ddx4 antibody
- DDX4_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of an Abreview submitted by Mai Sarrajab13840 at 1/1000 staining germ cells of the embryonic mouse testis by ICC/IF. Cells were PFA fixed and permeabilized in 1% Triton X in PBS prior to blocking in 5% serum for 2 hours at 20°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of an anonymous AbreviewAb13840 at 1/400 staining mouse fetal testis tissue sections by IHC-P. The tissue was blocked with both BSA and serum and fixed prior to incubation with the antibody for 3 hours at room temperature. A biotinylated secondary antibody was used (Vectastain Elite).
Please note Bouin's fluid was used as the fixative in this experiment.
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Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of Petra Hajkova, Gurdon Institute, University of CambridgeMouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1
µ The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
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Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 28 kDa, 47 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : ab13840 at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 52 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse Ovary Lysate at 10 µg
Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
Lane 3 : Mouse Ovary Lysate at 10 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg withDDX4 / MVH peptide (ab13841) at 1 µg/ml
Predicted band size: 76 kDa
Observed band size: 76 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of an Abreview submitted by Mr Kyle Cairesab13840 staining Postnatal Porcine Testis tissue sections by IHC-P. Bouin's Fixative was used to fix the sections prior to a heat mediated antigen retrieval step in sodium citrate buffer (pH 6). The sample was blocked in 10% serum for 1 hour at 22°C prior to incubation with the primary antibody, diluted 1/500, for 12 hours at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
References
This product has been referenced in:
- Becherel OJ et al. Disruption of Spermatogenesis and Infertility in Ataxia with Oculomotor Apraxia Type 2 (AOA2). Cerebellum 18:448-456 (2019). Read more (PubMed: 30778901) »
- Pham TH et al. Perinatal Exposure to Glyphosate and a Glyphosate-Based Herbicide Affect Spermatogenesis in Mice. Toxicol Sci 169:260-271 (2019). Read more (PubMed: 30785197) »
