• Product name
  • Description
    Rabbit polyclonal to DFFB
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human DFFB.

    (Peptide available as ab89389.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; K562; HeLa; HeLa Nuclear.



Our Abpromise guarantee covers the use of ab69438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
  • Sequence similarities
    Contains 1 CIDE-N domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD antibody
    • Caspase activated deoxyribonuclease antibody
    • Caspase activated DNase antibody
    • Caspase activated nuclease antibody
    • Caspase-activated deoxyribonuclease antibody
    • Caspase-activated DNase antibody
    • Caspase-activated nuclease antibody
    • CPAN antibody
    • Deoxyribonuclease III, caspase activated antibody
    • DFF 40 antibody
    • DFF-40 antibody
    • DFF2 antibody
    • DFF40 antibody
    • Dffb antibody
    • DFFB_HUMAN antibody
    • Didff antibody
    • DNA fragmentation factor 40 kDa subunit antibody
    • DNA fragmentation factor subunit beta antibody
    • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
    • DNA fragmentation factor, 40 kD beta subunit antibody
    • DNA fragmentation factor, 40 kD, beta polypeptide antibody
    • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
    • OTTHUMP00000003633 antibody
    see all


  • All lanes : Anti-DFFB antibody (ab69438) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa
    Additional bands at: 15 kDa (possible isoform), 30 kDa (possible isoform), 75 kDa, 77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 15 minutes

    This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3 and 4) of DNA fragmentation factor subunit beta (DFFB). The predicted molecular weights of isoforms (1,2,3 and 4) are 39 kDa, 30 kDa, 12 kDa and 11 kDa respectively.
  • ICC/IF image of ab69438 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69438, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and MCF-7 cells at 5µg/ml.


ab69438 has not yet been referenced specifically in any publications.

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