Anti-DIAPH1 antibody (ab11173)
Key features and details
- Rabbit polyclonal to DIAPH1
- Suitable for: IHC-P, ICC/IF, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIAPH1 antibody
See all DIAPH1 primary antibodies -
Description
Rabbit polyclonal to DIAPH1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Gorilla, Orangutan -
Immunogen
Synthetic peptide within DIAPH1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60610 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 24302570
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WB | (2) |
1/5000 - 1/15000. Predicted molecular weight: 141 kDa.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 24302570 |
WB
1/5000 - 1/15000. Predicted molecular weight: 141 kDa. |
Target
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Function
Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. -
Tissue specificity
Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. -
Involvement in disease
Defects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
Domain
DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain. -
Cellular localization
Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells. - Information by UniProt
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Database links
- Entrez Gene: 1729 Human
- Entrez Gene: 13367 Mouse
- Omim: 602121 Human
- SwissProt: O60610 Human
- SwissProt: O08808 Mouse
- Unigene: 529451 Human
- Unigene: 195916 Mouse
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Alternative names
- DIAPH1 antibody
- deafness, autosomal dominant 1 antibody
- DFNA1 antibody
see all
Images
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All lanes : Anti-DIAPH1 antibody (ab11173) at 0.66 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 141 kDa
Exposure time: 1 second -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DIAPH1 with ab11173 at 1/1000 (1µg/ml). Detection: DAB.
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IHC image of ab11173 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11173, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab11173 has been referenced in 10 publications.
- Palander O et al. Nonredundant roles of DIAPHs in primary ciliogenesis. J Biol Chem 296:100680 (2021). PubMed: 33872598
- Heib T et al. RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes. Cell Rep 35:109102 (2021). PubMed: 33979620
- Choltus H et al. Cigarette Smoke Condensate Exposure Induces Receptor for Advanced Glycation End-Products (RAGE)-Dependent Sterile Inflammation in Amniotic Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34361111
- Palander O & Trimble WS DIAPH1 regulates ciliogenesis and trafficking in primary cilia. FASEB J 34:16516-16535 (2020). PubMed: 33124112
- Swystun LL et al. The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity. J Clin Invest 128:4057-4073 (2018). PubMed: 30124466
- Manigrasso MB et al. Deletion of the formin Diaph1 protects from structural and functional abnormalities in the murine diabetic kidney. Am J Physiol Renal Physiol 315:F1601-F1612 (2018). PubMed: 30132346
- Lin YN et al. Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Oncotarget 6:18577-89 (2015). PubMed: 26124177
- Lin YN et al. Expression of DIAPH1 is up-regulated in colorectal cancer and its down-regulation strongly reduces the metastatic capacity of colon carcinoma cells. Int J Cancer 134:1571-82 (2014). PubMed: 24105619
- Araki K et al. Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization. Elife 2:e01228 (2013). IHC-Fr ; Human . PubMed: 24302570
- Ding Z et al. Silencing profilin-1 inhibits endothelial cell proliferation, migration and cord morphogenesis. J Cell Sci 119:4127-37 (2006). PubMed: 16968742