Overview

  • Product name
  • Description
    Rabbit polyclonal to DNAJB6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 126 of Human DNAJB6 (NP_490647).

  • Positive control
    • HeLaS3 and MOLT4 cell lysates for WB and HeLa cells for IF

Properties

Applications

Our Abpromise guarantee covers the use of ab96539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
  • Tissue specificity
    Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
  • Involvement in disease
    Limb-girdle muscular dystrophy 1E
  • Sequence similarities
    Contains 1 J domain.
  • Domain
    The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
  • Cellular localization
    Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJ4 antibody
    • DnaJ antibody
    • DnaJ (Hsp40) homolog subfamily B member 6 antibody
    • DNAJ B6 antibody
    • DnaJ homolog subfamily B member 6 antibody
    • DnaJ like 2 protein antibody
    • DNAJB6 antibody
    • DNJB6_HUMAN antibody
    • Heat shock protein J2 antibody
    • HHDJ1 antibody
    • HSJ-2 antibody
    • HSJ2 antibody
    • LGMD1E antibody
    • MRJ antibody
    • MSJ-1 antibody
    • MSJ1 antibody
    see all

Images

  • MDA-MB-231 whole cell extract (30 µg) separated by 12% SDS-PAGE. Anti-DNAJB6 antibody (ab96539) at 1/500 dilution.

  • ab96539, at 1/200, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. It would be very helpful if you could complete a questionnaire so that we can gather some further information regarding the samples tested and the protocol used. If you could please fill in both the WB and ICC questionnaires which i have attached to this email, this should only take five minutes per questionnaire. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up