Recombinant Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22845-83] to DPT/TRAMP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-DPT/TRAMP antibody [EPR22845-83]
See all DPT/TRAMP primary antibodies -
Description
Rabbit monoclonal [EPR22845-83] to DPT/TRAMP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skin, Mouse skin, Rat skin, Human testis, Human liver and Human pancreas lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22845-83 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
Notes |
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WB
1/1000. |
Target
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Function
Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation. -
Tissue specificity
Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts. -
Sequence similarities
Belongs to the dermatopontin family. -
Post-translational
modificationsSulfated on tyrosine residue(s). -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1805 Human
- Entrez Gene: 56429 Mouse
- Entrez Gene: 289178 Rat
- Omim: 125597 Human
- SwissProt: Q07507 Human
- SwissProt: Q9QZZ6 Mouse
- Unigene: 80552 Human
- Unigene: 28935 Mouse
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Alternative names
- DERM_HUMAN antibody
- Dermatopontin antibody
- Dpt antibody
see all
Images
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All lanes : Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human liver lysate
Lane 3 : Human pancreas lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Observed band size: 22 kDa why is the actual band size different from the predicted?Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate. There is weak expression in human testis, liver and pancreas. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10233760, 8082810).
Exposure time: 125 seconds
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All lanes : Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rat skin lysate
Lane 4 : Mouse skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Observed band size: 22 kDa why is the actual band size different from the predicted?The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10233760 and 8082810).
Negative control: HepG2 (PMID: 19916908).
Exposure time:
Lanes 1-3:15 seconds; Lanes 4:59 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab255823 has been referenced in 1 publication.
- Liu S et al. Dermatopontin inhibits WNT signaling pathway via CXXC finger protein 4 in hepatocellular carcinoma. J Cancer 11:6288-6298 (2020). PubMed: 33033513