• Product name
    Anti-Dicer antibody [13D6] - ChIP Grade
    See all Dicer primary antibodies
  • Description
    Mouse monoclonal [13D6] to Dicer - ChIP Grade
  • Host species
  • Specificity
    We do not guarantee its use in WB with mouse lysates.
  • Tested applications
    Suitable for: ICC, IP, ICC/IF, IHC-P, WB, ChIP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Caenorhabditis elegans
  • Immunogen

    Synthetic peptide corresponding to Human Dicer.

  • Positive control
    • WB: 10ng of recombinant dicer (using ECL or ECL Plus) in under one minute of exposure to film. ICC: Human Fibroblasts
  • General notes

    To our knowledge the localization of Dicer remains to be fully determined and it appears that its localization can be nuclear and/or cytoplasmic. Please see reference Meltzer, 2005 for a reference to a cytoplasmic localisation (figure 1 of paper), as supported with the staining seen in the image on this datasheet.
    Please note this antibody gave negative results in WB with mouse fibroblast cell line lysates. We do not guarantee its use in WB with mouse lysates.



Our Abpromise guarantee covers the use of ab14601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 20972213
ICC 1/20 - 1/100.
IP Use at an assay dependent concentration. PubMed: 24668803
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 18327211
WB 1/100 - 1/2000.

Please note this antibody gave negative results in WB with mouse fibroblast cell line lysates. We do not guarantee its use in WB with mouse lysates.

ChIP Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Function
    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • Involvement in disease
    Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • Sequence similarities
    Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DCR antibody
    • DCR1 antibody
    • Dicer 1 ribonuclease III antibody
    • Dicer 1 ribonuclease type III antibody
    • Dicer antibody
    • DICER_HUMAN antibody
    • DICER1 antibody
    • Double-strand-specific ribonuclease antibody
    • Endoribonuclease Dicer antibody
    • Helicase MOI antibody
    • Helicase with RNase motif antibody
    • HERNA antibody
    • KIAA0928 antibody
    • MNG1 antibody
    • RMSE2 antibody
    see all


  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Dicer knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab14601 observed at 240 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab14601 was shown to specifically react with Dicer in wild-type HAP1 cells. No band was observed when Dicer knockout samples were used. Wild-type and Dicer knockout samples were subjected to SDS-PAGE. ab14601 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 1/2000 dilution (incubated overnight at 4°C.)

    Lane 1 : NIH 3T3
    Lane 2 : A549
    Lane 3 : HepG2

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat anti-Mouse Green at 1/10000 dilution

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab14601 overnight at 4°C. Antibody binding was detected using IR-labelled goat anti-mouse Ab at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Overlay histogram showing HEK293 cells stained with ab14601 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14601, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • HepG2 cell lysate at 20 µg
    Developed using the ECL technique.

    Performed under reducing conditions.

    Additional bands at: 100 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)

  • ChIP analysis of Dicer protein binding at chicken ß-globin regulatory elements. Chromatin from human erythroleukemia (K562) cells containing chicken chromosomes with normal (FRK2) or mutant (HS1-K) ß-globin loci and chicken DT40 cells was immunprecipitated with antibodies to Dicer (ab14601). PCR analysis of immunoprecipitated chromatin was carried out using primers to the chicken ß-globin regulatory elements. Mouse IgG served as control. 10% of input DNA was utilized.

  • All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 2.5 µg/ml

    Lane 1 : human B cell lymphoma lysate
    Lane 2 : human B cell lymphoma lysate infected with control shRNA retrovirus
    Lane 3 : human B cell lymphoma lysate infected with Dicer knockdown shRNA virus

    Lysates/proteins at 2.5 µg/ml per lane.

    Observed band size: 225 kDa
    why is the actual band size different from the predicted?


This product has been referenced in:
  • Kong Q  et al. Endo-siRNAs regulate early embryonic development by inhibiting transcription of long terminal repeat sequence in pig. Biol Reprod N/A:N/A (2019). Read more (PubMed: 30883641) »
  • Kuscu C  et al. tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner. RNA 24:1093-1105 (2018). Read more (PubMed: 29844106) »
See all 105 Publications for this product

Customer reviews and Q&As

1-10 of 47 Abreviews or Q&A

Western blot
Hamster Cell lysate - whole cell (VeroE6 (Kidney from African green monkey), BHK21 ()
Gel Running Conditions
Reduced Denaturing (4-12% NuPage gel, MES buffer)
Loading amount
25000 cells
VeroE6 (Kidney from African green monkey), BHK21 (
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jul 11 2016

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Human Cell (Hela)
Yes - 0.05% tritonx-100

Dr. santosh chauhan

Verified customer

Submitted Apr 14 2014


The peptide immunogen was xxxxxxxx which corresponds to xxxxxxxx of the immature unprocessed sequence.

I would therefore suggest you use the following blocking peptide: Dicer peptide (ab24556)

Read More
Western blot
Mouse Tissue lysate - whole (Mammary Tumor)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
60 µg
Mammary Tumor
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 31 2013

Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (8%)
Rat Tissue lysate - whole (Mammary Tumor)
Mammary Tumor
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 30 2013


I am very pleased to hear you would like to test ab14601 in rat. This code will give you $379 off your next order before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Acording to UniProt, http://www.uniprot.org/uniprot/Q9UPY3, there are 2 isoforms of the Human Endoribonuclease Dicer (Gene name = Dicer1).
The isoform 2, also known as t-Dicer, differs from the canonical sequence as follows: 1789-1922: LRRSEEDEEK...KANQPQVPNS → KSFLQMYPVP...TTGRSESLWK.
The anti-Dicer ab14601, targeting Dicer specifically in the region between amino acids 1239 and 1255 of the PAZ domain, can therefore bind to both isoforms described on http://www.uniprot.org/uniprot/Q9UPY3.

Read More


Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the last 2 vials of this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results from the last 2 vials have not been successful.

I traced the order number for the two vials that did not work:

Order ##### Lot number GR97114-5
Order ##### Lot number GR97114-8

These were indeed from the same original lot number (GR97114). Unfortunately, I checked our system and I am very sorry to confirm we regrettably do not have any different lots in stock at the moment.

I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received bad vials on this occasion. Of course your enquiry will remain on our complaint records for continuing monitoring.

Therefore, I would be pleased to provide two replacement vials from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and if you prefer a credit note in this case, I will be pleased to arrange this for you.

I am sorry I have still not managed to trace your current order. I have alerted our customer service team to your request and asked them to let me know if they see the order coming through. However, we do have many orders coming through. Please let me know how you would like to proceed with this order, if you want to go ahead or cancel.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More


Good day, I do not have a problem so much as a technical question about the DICER1 ab14601 monoclonal antibody. The technical datasheet specifies that the antibody is raised against a synthetic peptide with no further details about the peptide itself. While I understand the specific peptide sequence is likely proprietary, is there any additional info you could provide to me about the region of the protein targeted by the antibody? The reason I would like to know is that we are using the antibody on human tumors from patients who have germline DICER1 mutations and, sometimes, somatic mutations on the second allele in the tumor only. The mutations occur in a variety of locations in the protein and it is unclear what protein products should be expected, if any. Because we are dealing with patients there isn't always material available for testing beyond FFPE tumors, which limits the methods that can be used to answer our questions at the protein level. The antibody seems to work really weell and often gives beautiful, apparently specific staining. However, we have some tumors that stain positive and others that stain negative, and the results don't always appear consistent with what we think we know of the germline and somatic mutation status of the tumors, so there are concerns about the specificity of the antibody to recognize our mutant proteins. We are hoping that if we knew which part of the protein is being recognized by the antibody, it might help sort out our apparent contradictory results or formulate logical hypotheses to test and answer reviewers' questions. Thank you for any assistance you can provide.

Read More

Thank you for contacting Abcam.
The peptide used to generate this antibody was located between amino acids *****of the human form of the protein (SwissProt: Q9UPY3 Human).
Please let me know if there is anything else I can help you with, Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Thank you very much for your call today and for letting us know about the trouble with this antibody.

Here areour suggestions for transferring high molecular weight proteins (>100 kDa):

For large proteins, transfer out of the gel may be very slow, just as they run slowly within the gel during separation. If blotting a large protein, be sure to run your samples in a low-concentration gel, 8% or less. These will be very fragile, so handle carefully.
Large proteins will tend to precipitate in the gel, hindering transfer. Adding SDS to a final concentration of 0.1% in the transfer buffer will discourage this. Methanol tends to remove SDS from proteins, so reducing the methanol percentage to 10% or less will also guard against precipitation.
Lowering methanol in the transfer buffer also promotes swelling of the gel, allowing large proteins to transfer more easily.
Methanol is only necessary if using nitrocellulose. If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich.
Choose wet transfer overnight at4°C instead of semi-dry transfer.

Generally for an overnight transfer, manufacturers recommend 10-30V depending on the equipment that you have. If you'd like to send me the name of the transfer equipment you use, I'll be happy to look up the specific suggestion (I've attached the BioRad guide, see page 24 if you're using one of their transfer boxes).

I hope that this information will be useful, but we do fully guarantee this antibody to work in your assay, so if the problem persists then I would be happy to send a replacement or issue a credit or refund. Please keep me updated about the results, and let me know if there is anything else that we can do for you.

Have a nice day!

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1-10 of 47 Abreviews or Q&A

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