Anti-Dicer antibody (ab228506)
Key features and details
- Chicken polyclonal to Dicer
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgY
Overview
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Product name
Anti-Dicer antibody
See all Dicer primary antibodies -
Description
Chicken polyclonal to Dicer -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse Dicer aa 1350-1450 conjugated to keyhole limpet haemocyanin.
Database link: Q8R418 -
Positive control
- WB: Mouse brain cytosolic extract.
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General notes
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Properties
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Form
Lyophilized:Reconstitute in 100 µl of sterile distilled water. Centrifuge to remove any insoluble material. After reconstitution, aliquot and store at -20°C for a higher stability or at 4°C with an appropriate antibacterial agent. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein L purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/3000. Predicted molecular weight: 217 kDa.
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Notes |
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WB
1/1000 - 1/3000. Predicted molecular weight: 217 kDa. |
Target
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Function
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. -
Involvement in disease
Pleuropulmonary blastoma
Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
Rhabdomyosarcoma, embryonal, 2
DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960). -
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 192119 Mouse
- SwissProt: Q8R418 Mouse
- Unigene: 21135 Mouse
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Alternative names
- DCR antibody
- DCR1 antibody
- Dicer 1 ribonuclease III antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228506 has not yet been referenced specifically in any publications.