Anti-Dnmt3L antibody (ab3493)
Key features and details
- Rabbit polyclonal to Dnmt3L
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3L antibody
See all Dnmt3L primary antibodies -
Description
Rabbit polyclonal to Dnmt3L -
Host species
Rabbit -
Specificity
This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Dnmt3L aa 152-164 conjugated to keyhole limpet haemocyanin.
Sequence:C-GLLQRRRKWRSQL
Database link: Q9UJW3 -
General notes
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested. -
Primary antibody notes
DNMT 3L is a nuclear protein which has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | (6) |
1/100.
|
Notes |
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IP
1/100. |
Target
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Function
Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. -
Tissue specificity
Expressed at low levels in several tissues including testis, ovary, and thymus. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29947 Human
- Omim: 606588 Human
- SwissProt: Q9UJW3 Human
- Unigene: 592165 Human
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Alternative names
- Cytosine 5 methyltransferase 3 like protein antibody
- DNA (cytosine 5 ) methyltransferase 3 like antibody
- DNA (cytosine-5)-methyltransferase 3-like antibody
see all
Images
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Transfection of U2OS cells (10cm dish) using 3
µ g of GAL4-DNMT3L. Protein extraction using IPH 150mM. IP and WB conditions, see Fuks et al. (2000). Nature Genetics 24: 88-91.WB using 0.4µ g/ml in TBS milk 2%, BSA 0.5%. Dectection using ECL.Note: ab3493 worked in WB following IP, but did not work in a straight WB.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab3493 has been referenced in 15 publications.
- Uysal F et al. DNMT enzymes differentially alter global DNA methylation in a stage-dependent manner during spermatogenesis. Andrologia 54:e14357 (2022). PubMed: 34997784
- Wang Y et al. Retinoic acid induces NELFA-mediated 2C-like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media. Cell Prolif 54:e13049 (2021). PubMed: 33960560
- Wu B et al. Activin A and BMP4 Signaling Expands Potency of Mouse Embryonic Stem Cells in Serum-Free Media. Stem Cell Reports 14:241-255 (2020). PubMed: 32032551
- Ishihara T et al. DNA methylation dynamics in the germline of the marsupial tammar wallaby, Macropus eugenii. DNA Res 26:85-94 (2019). PubMed: 30535324
- Peng J et al. The detrimental effects of glucocorticoids exposure during pregnancy on offspring's cardiac functions mediated by hypermethylation of bone morphogenetic protein-4. Cell Death Dis 9:834 (2018). PubMed: 30082698