• Product name
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 488)
  • Host species
  • Target species
  • Specificity
    This antibody is specific to Goat IgG
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
  • Isotype
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab150129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H+L) (shown in green) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Overlay histogram showing Jurkat cells stained Goat polyclonal to Ikaros (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Goat polyclonal to Ikaros, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 donkey anti-goat IgG (H&L) (ab150129) at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was goat IgG (polyclonal) (ab37373, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


This product has been referenced in:
  • Lv Y  et al. Myosin IIA Regulated Tight Junction in Oxygen Glucose-Deprived Brain Endothelial Cells Via Activation of TLR4/PI3K/Akt/JNK1/2/14-3-3e/NF-?B/MMP9 Signal Transduction Pathway. Cell Mol Neurobiol 39:301-319 (2019). Read more (PubMed: 30666520) »
  • Yao X  et al. Deferoxamine promotes recovery of traumatic spinal cord injury by inhibiting ferroptosis. Neural Regen Res 14:532-541 (2019). Read more (PubMed: 30539824) »
See all 84 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
This antibody was used to study the TIMP1-expression in human skin tissue (melanoma vs nevus) by indirect immunfluorescence.
The anti goat antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results.
Concentration 1:200

Abcam user community

Verified customer

Submitted Mar 04 2019

Immunohistochemistry (PFA fixed)
Very good antibody

Yacine Tensaouti

Verified customer

Submitted Aug 01 2017


For excitation with the 543nm laser, Alexa Fluor 568 is a better choice than 555 for combination with AF488. There is still some emission spillover but it is much less than for the 488/555 combination, and you should be able to set compensation without as much trouble. If the 488 signal is expected to be much brighter than the other, for instance if that protein is much more abundant than the other, then spillover may still be an issue. For an anti-mouse IgG secondary, I suggest ab175473.

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Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab150129. I would also appreciate if you can confirm some further details: Do you use a detergent such as Tween 20 in your washing buffer? Have you attempted to increase the number and duration of washes after antibody incubation? If so, did this have affect on the background? Have you attempted to further dilute the antibody to 1/1500, 1/2000 or greater? This antibody has not been pre-absorbed against mouse IgG, as such we cannot guarantee that we will be able to eliminate this reactive thought we hope that these suggestions will help reduce this. Abcam does offer a number of AlexaFluor 488 antibodies which have been pre-absorbed to ensure low species cross-reactivity including ab150133 which has been pre-absorbed against chicken, human, mouse, pig, rabbit and rat. More details about this product may be found at: https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150133.html

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Immunocytochemistry/ Immunofluorescence
Following fixation in 4% PFA, the cells were assessed for Brachyury (T) expression using 1:100 dilution of the primary antibody in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using donkey polyclonal rabbit IgG Alexa 488 (ab150129) at 1:500. The results show that nuclear T (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here). All nuclei were counterstained using DAPI (blue).

Abcam user community

Verified customer

Submitted Sep 02 2013

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