Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab97112)
Key features and details
- Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Human, Mouse, Pig, Rabbit, Rat more details -
Immunogen
Other Immunogen Type corresponding to Goat IgG.
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/100 - 1/1000.
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ELISA |
1/2500 - 1/25000. (as Primary).
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WB |
1/2500 - 1/25000. (Chemiluminescent).
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/100 - 1/1000. |
ELISA
1/2500 - 1/25000. (as Primary). |
WB
1/2500 - 1/25000. (Chemiluminescent). |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab97112 has been referenced in 2 publications.
- Hasegawa C et al. Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads. J Vet Med Sci 82:414-421 (2020). PubMed: 32092744
- Yamamoto A et al. The mechanisms underlying the effects of AMH on Müllerian duct regression in male mice. J Vet Med Sci 80:557-567 (2018). PubMed: 29526868