Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details -
Minimal
cross-reactivity
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
Immunogen
Full length native Goat IgG (purified).
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Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
Conjugation notes
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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ELISA |
1/10000 - 1/50000.
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
WB
1/1000. Predicted molecular weight: 36 kDa. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab6949 has been referenced in 41 publications.
- Saga Y et al. The cytosolic lncRNA dutA affects STATa signaling and developmental commitment in Dictyostelium. Genes Cells 28:111-128 (2023). PubMed: 36504347
- Xu Y et al. Caffeine Functions by Inhibiting Dorsal and Ventral Hippocampal Adenosine 2A Receptors to Modulate Memory and Anxiety, Respectively. Front Pharmacol 13:807330 (2022). PubMed: 35185566
- Chang P et al. Fully Reduced HMGB1-Containing Peptide-Based Polyurethane Scaffold with Minimal Functional Unit of Skin (MFUS) Enhances Large and Deep Wounded Skin Healing. Macromol Biosci 22:e2100403 (2022). PubMed: 34783444
- Ji J et al. Bu Shen Yi Sui Capsules Promote Remyelination by Regulating MicroRNA-219 and MicroRNA-338 in Exosomes to Promote Oligodendrocyte Precursor Cell Differentiation. Evid Based Complement Alternat Med 2022:3341481 (2022). PubMed: 35463062
- Tseng KY et al. Sciatic Nerve Intrafascicular Injection Induces Neuropathy by Activating the Matrix Modulators MMP-9 and TIMP-1. Front Pharmacol 13:859982 (2022). PubMed: 35694244