Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Specificity
    This antibody is specific to Mouse IgG
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab150105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

Images

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150105 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

References

This product has been referenced in:
  • Jayasuriya CT  et al. Human Cartilage-Derived Progenitors Resist Terminal Differentiation and Require CXCR4 Activation to Successfully Bridge Meniscus Tissue Tears. Stem Cells 37:102-114 (2019). Read more (PubMed: 30358021) »
  • Pajokh M  et al. Apoptosis, Autophagy, and Necrosis in Murine Embryonic Gonadal Ridges and Neonatal Ovaries: An Animal Model. Iran J Med Sci 44:35-43 (2019). Read more (PubMed: 30666074) »
See all 63 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Worked very well against Mouse primary antibody against RUNX2

Abcam user community

Verified customer

Submitted Jun 11 2018

Abreviews
Application
Immunocytochemistry
Sample: Human normal fibloblast cells
Treatment: 4% formalin fixation, 0.1% Triton-X100 permeabilization
1st antibody: anti-vimentin mouse monoclonal antibody
2nd antibody: anti-mouse IgG Alexa-488 (ab150105)
Mount: VECTASHIELD Mounting Medium with DAPI

Result: Specific staining with low background.

Abcam user community

Verified customer

Submitted Aug 22 2017

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Excellent antibody. Used at 1:500 dilution in PBS-Triton X-100 (0.1%) overnight at 4 degrees. Used in conjunction with GFAP antibody (Ab80842). Stable fluorescence when mounted with Vectashield (inc DAPI).

Dr. Craig Beall

Verified customer

Submitted Sep 08 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This antibody works perfectly at a 1:500 dilution with SALL4 primary antibody (ab57577, mouse monoclonal). However, the intensity of this Donkey secondary antibody is weaker than that of the Goat secondary antibody (ab150113) at the same dilution. So that is why I gave it 4 stars.

Dr. 煜欽 林

Verified customer

Submitted Oct 31 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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