Donkey Anti-Mouse IgG H&L (FITC) preadsorbed (ab7057)
Key features and details
- Donkey Anti-Mouse IgG H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Donkey
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr
Overview
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Product name
Donkey Anti-Mouse IgG H&L (FITC) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native mouse whole (purified).
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/2500.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
Flow Cyt |
1/2000 - 1/10000.
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|
IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/500 - 1/2500. |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/2000 - 1/10000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (7)
ab7057 has been referenced in 7 publications.
- Jin S et al. Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke. iScience 26:105885 (2023). PubMed: 36654858
- Feng Z et al. Heavy-ion beam irradiation inhibits invasion of tongue squamous cell carcinoma Tca8113 cells. Oncol Lett 18:4092-4099 (2019). PubMed: 31516609
- Lindén S et al. Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes. Dalton Trans 44:4994-5003 (2015). PubMed: 25612290
- Ataide MA et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014). PubMed: 24453977
- Radu BM et al. Dual effect of methylglyoxal on the intracellular Ca2+ signaling and neurite outgrowth in mouse sensory neurons. Cell Mol Neurobiol 32:1047-57 (2012). ICC/IF . PubMed: 22402835
- Miranda E et al. The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB. Hum Mol Genet 17:1527-39 (2008). PubMed: 18267959
- Griffin S et al. Astrocyte-neurone communication following oxygen-glucose deprivation. J Neurochem 95:1015-22 (2005). PubMed: 16190880