Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (ab98799)
Key features and details
- Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
Overview
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Product name
Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: IHC-P, ELISA, WB, ICCmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. Primary dilution.
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WB |
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution.
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|
ICC |
Use at an assay dependent dilution.
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Notes |
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IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. Primary dilution. |
WB
1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution. |
ICC
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab98799 has been referenced in 8 publications.
- Fukushi M et al. Optineurin deficiency impairs autophagy to cause interferon beta overproduction and increased survival of mice following viral infection. PLoS One 18:e0287545 (2023). PubMed: 37352136
- Hinden L et al. Opposite physiological and pathological mTORC1-mediated roles of the CB1 receptor in regulating renal tubular function. Nat Commun 13:1783 (2022). PubMed: 35379807
- Pandey SK et al. Silencing VDAC1 to Treat Mesothelioma Cancer: Tumor Reprograming and Altering Tumor Hallmarks. Biomolecules 12:N/A (2022). PubMed: 35883451
- Baraghithy S et al. Renal Proximal Tubule Cell Cannabinoid-1 Receptor Regulates Bone Remodeling and Mass via a Kidney-to-Bone Axis. Cells 10:N/A (2021). PubMed: 33671138
- Pittala S et al. The VDAC1-based R-Tf-D-LP4 Peptide as a Potential Treatment for Diabetes Mellitus. Cells 9:N/A (2020). PubMed: 32093016
- Azar S et al. Reversal of diet-induced hepatic steatosis by peripheral CB1 receptor blockade in mice is p53/miRNA-22/SIRT1/PPARa dependent. Mol Metab 42:101087 (2020). PubMed: 32987186
- Drori A et al. CB1R regulates soluble leptin receptor levels via CHOP, contributing to hepatic leptin resistance. Elife 9:N/A (2020). PubMed: 33210603
- Yannai S et al. Combinatorial treatment increases IKAP levels in human cells generated from Familial Dysautonomia patients. PLoS One 14:e0211602 (2019). PubMed: 30889183