Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

     

  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919) was used as a secondary antibdoy at a dilution of 1/50.
  • E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

    This image is courtesy of an anonymous Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Pérès EA  et al. Longitudinal Study of Irradiation-Induced Brain Microstructural Alterations With S-Index, a Diffusion MRI Biomarker, and MR Spectroscopy. Int J Radiat Oncol Biol Phys 102:1244-1254 (2018). IHC-Fr . Read more (PubMed: 29680253) »
  • Rodriguez AM & Downs KM Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula. Dev Biol N/A:N/A (2017). Read more (PubMed: 28882402) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Tal y como hemos quedado, he creado un código de descuento: XXXXXX para testar ab5794 en células de gato mediante ICC.

El código caduca el día 20-Marzo-2013.

Este código tiene un valor de €370 si se realiza la compra antes de la fecha de caducidad. Para beneficiarse de esta oferta, por favor, mándanos los resultados mediante una Abreview obtenidos al testar el anticuerpo. Incluye por favor en los “Additional Comments” el código indicado, para que nosotros sepamos a qué corresponde dicha publicación.

Para más información acerca de publicar un Abreview, por favor, visita la página: https://www.abcam.com/Abreviews

Te recuerdo que publicamos resultados tanto positivos como negativos en las datasheet de los productos. El código se activará una vez que se haya publicado el Abreview, y puede canjearse mencionando el código de descuento proporcionado a la hora de hacer el pedido, bien sea por teléfono, por fax, o por email. ES IMPRESCINDIBLE INDICAR DICHO CODIGO PARA PODER BENEFICIARSE DEL DESCUENTO.

Las condiciones y términos de esta oferta las encontrarás en:

https://www.abcam.com/abtrial

En el pedido que hagas ahora, indica el código del anticuerpo primario (ab5794) el secundario que deseas adquirir (ab96919), y el del anticuerpo gratuito Rabbit monoclonal.

Me temo que el que me indicas en el mail no forma parte de la promoción, sin embargo, https://www.abcam.com/netrin-1-antibody-epr5428-ab126729.html también reconoce Netrin 1 y sí forma parte de la promoción. Si te inclinas finalmente por éste, indica su código en el pedido así como el código de la promoción para que se descuente su importe (RABMAB-XBSMG).

Si quieres que te envíe yo directamente el presupuesto con los tres productos, aplicando el descuento, indícamelo y estaré encantada de hacerlo.

Si tienes cualquier duda, por favor no dudes en contactarnos.

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Answer

Thank you for contacting us.

I think this information would need to be obtained from the manufacturer of the microscope and filters that the customer is planning touse. They would be able to exactly tell the customer which wavelengths can be detected with these 2 filters.

The filter I 450-490 FT510 LP520 would probably work with the for 488 secondary antibody (ab96919), but I am not certain about the second one. The manufacturer would also be able to tell what other filters to use to make this work for the 594 secondary antibody (ab98768).

The numbers 488 and 594 refer to the excitation wavelength of the fluorophore. The emission wavelength are 519 nm (for the 488) and 617 nm (for the 594 fluorophore).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for your call.

As discussed on the phone, the ab96919 can be used in a variety of protocols and applications. Please find here also the links to our online immunohistochemistry (IHC)protocols.

IHC protocol summary: https://www.abcam.com/index.html?pageconfig=resource&rid=11757

IHC frozen sections: https://www.abcam.com/index.html?pageconfig=resource&rid=11383

IHC paraffin staining protocol (detailed): https://www.abcam.com/index.html?pageconfig=resource&rid=11384

I hope this information is helpful. Please do not hesitate to call back, if you would like to discuss the protocols or the procedures in more detail.

I wish youa great day!

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Answer

DISCOUNT CODE: 42087
Expiration date: 28/05/2012
I am very pleased to hear you would like to accept our offer and test ab96919 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date.
To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry and your interest in our products.
I can certainly offer a discount code for testing this secondary antibody for IHC on frozen sections.
Could you please pass your customer's contact details for our internal record and I can process the code for you as soon as possible.
I look forward to hearing from you soon.

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Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

ab73430 (DNase I protein (Active))

https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Answer

Thank you for providing that extra information. Out of the secondaries used, the DyLight488 (ab96919) definitely seems to be producing more diffuse, non-nuclear staining compared to the other three. It may be that this could be reduced by optimising the blocking conditions and the antibody concentration. However, from the protocol you have used, I would expect the results to be better.

It may be that unfortunately you have received a faulty batch of this product. I am very sorry for the inconvenience this has caused.

If you would like, I can change the antibody for an alternative lot or I can offer an alternative antibody altogether (such as ab96895 or ab96899). These are both secondary antibodies to rabbit IgG with DyLight488 conjugation. If you would prefer a different conjugate I could accommodate this too. Alternatively I can provide a full refund.

In order to process any of these options I will need an order number on which the antibody was ordered (or an approximate date of the order and the delivery address).

I look forward to hearing how you would like to proceed.

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Answer

Thank you for taking time to complete our questionnaire. I am sorry to hear that the Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody (ab96919) is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to clarify a few points in order to understand the problems you have been having more fully.

1. You mention that the problem is non-specific staining, could you share the image obtained from the staining with me? Do you get the same staining with the "no-primary" control??

2. Do you have the catalogue number for the primary antibody used? Was this the same primary antibody used with the Cy2 and Alexa488 staining? Would you be able to share the images produced from these experiments?

These details will greatly help me to understand what may be going wrong.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. In order to process this I would need an order number or the date of the order and the delivery address in order to locate your order.

I hope this information is helpful, and I thank you for your cooperation.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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