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    dopamine-elisa-kit-ab285238.pdf

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Neuroscience Neurotransmitter Biogenic Amines Dopamine
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Dopamine ELISA Kit (ab285238)

  • Datasheet
  • SDS
  • Protocol Booklet
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Dopamine (DA) ELISA Kit

    Key features and details

    • Sensitivity: 0.938 ng/ml
    • Range: 1.56 ng/ml - 100 ng/ml
    • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Homogenate
    • Detection method: Colorimetric
    • Assay type: Quantitative

    Overview

    • Product name

      Dopamine ELISA Kit
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Homogenate
    • Assay type

      Quantitative
    • Sensitivity

      < 0.938 ng/ml
    • Range

      1.56 ng/ml - 100 ng/ml
    • Assay duration

      Multiple steps standard assay
    • Product overview

      Dopamine ELISA Kit (ab285238, K4219) is a competitive ELISA assay for the quantitative measurement of Dopamine in serum, plasma and cell culture supernatants. Dopamine is an organic chemical of the catecholamine and phenethylamine families that plays several important roles in the brain and body. In the brain, dopamine functions as a neurotransmitter. Outside the central nervous system, dopamine functions in several parts of the peripheral nervous system as a local chemical messenger. The concentration of Dopamine in the samples can be calculated by comparing the OD of the samples to the standard curve.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K4219 Dopamine (DA) ELISA Kit. K4219-100 is the same size as the 96 test size of ab285238.

    • Platform

      Microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 96 tests
      25X Wash buffer 1 x 30ml
      Antibody dilution buffer 1 x 10ml
      Biotin-Detection antibody (Concentrated) 1 x 60µl
      HRP-Streptavidin Conjugate (SABC) 1 x 120µl
      Lyophilized Standard 2 vials
      Micro ELISA Plate 1 unit
      Plate Sealer 5 units
      SABC dilution buffer 1 x 10ml
      Sample and Standard Dilution Buffer 1 x 20ml
      Stop Solution 1 x 10ml
      TMB Substrate 1 x 10ml
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Biogenic Amines
      • Dopamine
    • Relevance

      Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
    • Cellular localization

      Secreted
    • Alternative names

      • 3 4 dihydroxyphenethylamine
      • 3 hydroxytyramine
      • DA
      • Dopamine
      • Oxytyramine
      see all

    Images

    • Dopamine (DA) ELISA Kit
      Dopamine (DA) ELISA Kit

      Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab285238? Please let us know so that we can cite the reference in this datasheet.

    ab285238 has not yet been referenced specifically in any publications.

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