Anti-Dopamine beta Hydroxylase antibody (ab19353)
Key features and details
- Sheep polyclonal to Dopamine beta Hydroxylase
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat
- Isotype: n/a
Overview
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Product name
Anti-Dopamine beta Hydroxylase antibody
See all Dopamine beta Hydroxylase primary antibodies -
Description
Sheep polyclonal to Dopamine beta Hydroxylase -
Host species
Sheep -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Rat Dopamine beta Hydroxylase aa 43-62.
Sequence:SEPPESPFPYHIPLDPEGTL
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General notes
Detects human, bovine, rat and mouse DBH. It is not effective for immunohistochemistry in human brainThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/5000.
|
|
IHC-P |
1/2000.
|
Notes |
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ICC/IF
1/5000. |
IHC-P
1/2000. |
Target
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Function
Conversion of dopamine to noradrenaline. -
Pathway
Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1. -
Involvement in disease
Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. -
Sequence similarities
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
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Database links
- Entrez Gene: 25699 Rat
- SwissProt: Q05754 Rat
- Unigene: 87166 Rat
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Alternative names
- dbh antibody
- DBM antibody
- Dopamine beta hydroxylase antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab19353 has been referenced in 8 publications.
- Blokhin V et al. A New Method for the Visualization of Living Dopaminergic Neurons and Prospects for Using It to Develop Targeted Drug Delivery to These Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35409040
- Cui K et al. Restoration of Noradrenergic Function in Parkinson's Disease Model Mice. ASN Neuro 13:17590914211009730 (2021). PubMed: 33940943
- Kim SM et al. Repurposing erectile dysfunction drugs tadalafil and vardenafil to increase bone mass. Proc Natl Acad Sci U S A 117:14386-14394 (2020). PubMed: 32513693
- Luo Z et al. Effect of Electrical Stimulation of Cervical Sympathetic Ganglia on Intraocular Pressure Regulation According to Different Circadian Rhythms in Rats. Invest Ophthalmol Vis Sci 61:40 (2020). PubMed: 32976562
- Underhill SM et al. Amphetamine Stimulates Endocytosis of the Norepinephrine and Neuronal Glutamate Transporters in Cultured Locus Coeruleus Neurons. Neurochem Res 45:1410-1419 (2020). PubMed: 31912366
- Scott AL et al. Regulation of catecholamine release from the adrenal medulla is altered in deer mice (Peromyscus maniculatus) native to high altitudes. Am J Physiol Regul Integr Comp Physiol 317:R407-R417 (2019). PubMed: 31242021
- Mahata SK et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). PubMed: 27402067
- Talavera-Adame D et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). PubMed: 19364272