Anti-Dynamin 3 antibody (ab3458)
Key features and details
- Rabbit polyclonal to Dynamin 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Dynamin 3 antibody
See all Dynamin 3 primary antibodies -
Description
Rabbit polyclonal to Dynamin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat Dynamin 3 aa 623-639.
Sequence:PDKSFTENDENGQAENF
(Peptide available asab4986) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. By Western blot, this antibody detects an ~100 kDa protein representing Dynamin 3 from HeLa cell lysate.
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IHC-P |
Use a concentration of 4 µg/ml.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use at an assay dependent concentration. By Western blot, this antibody detects an ~100 kDa protein representing Dynamin 3 from HeLa cell lysate. |
IHC-P
Use a concentration of 4 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. -
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
Contains 1 GED domain.
Contains 1 PH domain. -
Cellular localization
Cytoplasm. Cytoplasm, cytoskeleton. Microtubule-associated. - Information by UniProt
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Database links
- Entrez Gene: 26052 Human
- Entrez Gene: 171574 Rat
- Omim: 611445 Human
- SwissProt: Q9UQ16 Human
- SwissProt: Q08877 Rat
- Unigene: 654775 Human
- Unigene: 11191 Rat
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Alternative names
- testicular dynamin antibody
- testicular antibody
- DNM 3 antibody
see all
Images
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Western blot of Dynamin 3 on rat liver extract using ab3458.
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ab3458 (4µg/ml) staining Dynamin 3 in human brain cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the white matter region
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ICC/IF image of ab3458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab3458 has been referenced in 11 publications.
- Flores-Muñoz C et al. The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal Neurons. Cells 11:N/A (2022). PubMed: 36429074
- Flores-Muñoz C et al. Restraint of Human Skin Fibroblast Motility, Migration, and Cell Surface Actin Dynamics, by Pannexin 1 and P2X7 Receptor Signaling. Int J Mol Sci 22:N/A (2021). PubMed: 33499026
- Ding W et al. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits. JCI Insight 3:N/A (2018). PubMed: 29563333
- Li Y et al. Transcriptomes of cochlear inner and outer hair cells from adult mice. Sci Data 5:180199 (2018). PubMed: 30277483
- Gu C et al. Dynamin 3 suppresses growth and induces apoptosis of hepatocellular carcinoma cells by activating inducible nitric oxide synthase production. Oncol Lett 13:4776-4784 (2017). PubMed: 28599479