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Mouse embryonal carcinoma cell line PCC4 Aza RI.
Our Abpromise guarantee covers the use of ab11512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20521328
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. PubMed: 19586906|
|IHC-Fr||Use at an assay dependent concentration.|
ab11512 stained in M158 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11512 at 10 µg/ml overnight at +4°C. The secondary antibody was ab150165 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
Sample: Murine derived breast cancer whole cell lysate, 30 µg.
ab11512 used at a 1/5000 dilution for 16 hours at 4°C.
Goat polyclonal IRDye 800CW used as the secondary antibody at a 1/10,000 dilution.
ab11512 staining E Cadherin in Mouse embryonic (E14.5) lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 3 hours at 4°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 14 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
ab11512 at 1/500 staining dog kidney cells by ICC/IF. The cells were methanol fixed and blocked with BSA before incubation with the antibody for 18 hours at 4°C. An Alexa Fluor ® 555 conjugated goat anti-rat IgG was used as the secondary.
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