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    e-cadherin-antibody-ep700y-intercellular-junction-marker-ab40772.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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RecombinantRabMAb

Recombinant Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

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Reviews (22)Q&A (6)References (464)

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Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Flow Cytometry (Intracellular) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Flow Cytometry (Intracellular) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • OI-RD Scanning - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
  • Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP700Y] to E Cadherin - Intercellular Junction Marker
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

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Alexa Fluor® 647 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab194982)
Protein
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Recombinant Human E Cadherin protein (ab235682)
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Indoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit (ab235936)

View more associated products

Overview

  • Product name

    Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker
    See all E Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EP700Y] to E Cadherin - Intercellular Junction Marker
  • Host species

    Rabbit
  • Specificity

    E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. This antibody has been tested on human samples in both WB and IHC. Customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human E Cadherin aa 600-700. The exact sequence is proprietary.
    Database link: P12830

  • Positive control

    • WB: Positive controls: MCF-7, HT-29, PC-3 whole cell lysate. Negative Control: MDA-MB-231 whole cell lysate IHC-P: Human breast carcinoma, lung adenocarcinoma and colonic adenocarcinoma tissue. Human papillary carcinoma of thyroid gland and transitional cell carcinoma of kidney tissue. ICC/IF: MCF7 and HT-29 cells. Flow Cyt (intra): A431 and MCF7 cells.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 2.80 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP700Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development
    • Signal Transduction
    • Antibodies
    • e cadherin
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab185013)
    • Alexa Fluor® 647 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab194982)
    • Anti-E Cadherin antibody [EP700Y] - Low endotoxin, Azide free (ab201499)
    • Alexa Fluor® 555 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab206878)
    • Alexa Fluor® 594 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab206880)
    • PE Anti-E Cadherin antibody [EP700Y] (ab224959)
    • APC Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab224960)
    • Anti-E Cadherin antibody [4A2] (ab231303)
    • Anti-E Cadherin antibody [EP700Y] - BSA and Azide free (ab256580)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human E Cadherin protein (ab235682)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-E Cadherin antibody [EPR699] (ab133597)
    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For purified format use at1/1000.

ICC/IF (9)
1/500.

Permeabilisation is unnecessary as the immunogen is in an extracellular domain.

IHC-P (4)
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB (5)
1/10000 - 1/50000. Detects a band of approximately 80-120 kDa (predicted molecular weight: 97 kDa).
Notes
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For purified format use at1/1000.

ICC/IF
1/500.

Permeabilisation is unnecessary as the immunogen is in an extracellular domain.

IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
1/10000 - 1/50000. Detects a band of approximately 80-120 kDa (predicted molecular weight: 97 kDa).

Target

  • Function

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • Tissue specificity

    Non-neural epithelial tissues.
  • Involvement in disease

    Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Post-translational
    modifications

    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • Cellular localization

    Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Target information above from: UniProt accession P12830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 999 Human
    • Omim: 192090 Human
    • SwissProt: P12830 Human
    • Unigene: 461086 Human
    • Alternative names

      • Arc 1 antibody
      • CADH1_HUMAN antibody
      • Cadherin 1 antibody
      • cadherin 1 type 1 E-cadherin antibody
      • Cadherin-1 antibody
      • Cadherin1 antibody
      • CAM 120/80 antibody
      • CD 324 antibody
      • CD324 antibody
      • CD324 antigen antibody
      • cdh1 antibody
      • CDHE antibody
      • E-Cad/CTF3 antibody
      • E-cadherin antibody
      • ECAD antibody
      • Epithelial cadherin antibody
      • epithelial calcium dependant adhesion protein antibody
      • LCAM antibody
      • Liver cell adhesion molecule antibody
      • UVO antibody
      • Uvomorulin antibody
      see all

    Images

    • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      All lanes : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/10000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
      Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell). Whole cell lysates
      Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) Whole cell lysates
      Lane 4 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (negative control)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Performed under reducing conditions.

      Predicted band size: 97 kDa


      Exposure time: 23 seconds


      Blocking and diluting buffer: 5% NFDM/TBST

      Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456

    • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)Omata, W. et al PLoS One. 2013 Nov 13;8(11):e81003. doi: 10.1371/journal.pone.0081003. eCollection 2013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      PMA induced cell fusion, DYSF expression, and activation of PKC in BeWo cells while 4αPMA was inactive

      Immunofluorescence analysis of BeWo cells treated with 0.25% DMSO (controls), 10 nM PMA, or 10 nM 4αPMA for 72 h. The cells were then fixed and subsequently double-labeled for detection of DYSF (red) and E-cadherin (green). Nuclei were labeled with DAPI. While there can be a low level of spontaneous fusion in control cells (in our hands this ranges from about 4 to 9%), most cells are not fused and have at their borders intact E-cadherin labeling. Moreover, DYSF labeling was not detectable in non-fused BeWo cells. However, treatment of BeWo cells with 10 nM PMA for 72 h led to increased levels of cell fusion as indicated by the breakdown of E-cadherin labeling and the expression of DYSF in fused cells. When BeWo cells were treated with 10 nM 4αPMA for 72 h there was no detectable increase in cell fusion or DYSF expression. Arrows indicate areas enlarged and placed in insets. Bar = 50 µm.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)Roca, H. et al PLoS One. 2013 Oct 4;8(10):e76773. doi: 10.1371/journal.pone.0076773. eCollection 2013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Mesenchymal cancer cells show increased metastasis while not requiring MET for solid tumor formation.

      ZEB1 or E-cadherin staining of metastases in ICI-mice. Note the higher E-cad and lower ZEB1 expression in the metastatic cells expressing OVOL1 or ZEB1-shRNA (sh4). Scale bar represents 100 µm.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      ab40772 staining E Cadherin in HT-29 (Human colorectal adenocarcinoma) cells by ICC/IF (Immunocytochemistry/Immunofluorescence).  Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at 1/500 dilution. An Alexa Fluor® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution was used as a counterstain. DAPI was used as a nuclear counterstain. This is a confocal image showing membranous staining on HT-29 cell line.

    • Flow Cytometry (Intracellular) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Flow Cytometry (Intracellular) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling E Cadherin with purified ab40772 at 1/30 dilution (10µg/ml) (red). 106 cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab40772. Green-E-Cadherin red-PI.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Flow Cytometry (Intracellular) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Overlay histogram showing A431 (Human epidermoid carcinoma cell line) cells stained with unpurifiedab40772 (red line).106 cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40772, 1/1000 dilution) for 30 minute at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Formalin/PFA-fixed paraffin-embedded human colonic adenocarcinoma tissue stained for E Cadherin with unpurified ab40772 at a 1/500 dilution in immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Lane 1 : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/5000 dilution
      Lane 2 : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/2000 dilution
      Lane 3 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

      All lanes : PC-3 (Human prostate adenocarcinoma epithelial cell) Whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa



      Exposure time: 3 minutes for ab40772 and ab133597, 32 seconds for GAPDH.

      Blocking and diluting buffer: 5% NFDM/TBST

      Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456

    • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial) cells labeling E Cadherin with ab40772. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were then incubated with the primary antibody at a 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at a 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Counterstained with ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).

      Confocal image shows membranous staining on MCF7 cell line.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for E Cadherin with unpurified ab40772 at a 1/500 dilution in immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Lane 1 : Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/5000 dilution
      Lane 2 : Anti-E Cadherin antibody [EPR699] (ab133597) at 1/2000 dilution
      Lane 3 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

      All lanes : HT-29 (Human colorectal adenocarcinoma epithelial cell). Whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Exposure time: 1 second for ab40772, 3 minutes for ab133597, 32 seconds for GAPDH

      Blocking and diluting buffer: 5% NFDM/TBST

      Multi-bands can refer to PMID: 11212238; PMID: 14695147 and PMID: 22659456

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for E Cadherin with unpurified ab40772 at a 1/500 dilution in immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/200000 dilution (Unpurified) + MCF7 (Human breast adenocarcinoma ) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

      Predicted band size: 97 kDa
      Observed band size: 100,120,80,97 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      The full-length of E-cadherin is 120 kDa. The other bands are due to proteolytic cleavages in different Cadherin domains. (Ref: PMID: 14695147)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Formalin-fixed, paraffin-embedded human papillary carcinoma of thyroid gland tissue stained for E Cadherin with unpurified ab40772 at a 1/500 dilution in immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Formalin-fixed, paraffin-embedded human transitional cell carcinoma of kidney tissue stained for E Cadherin with unpurified ab40772 at a 1/500 dilution in immunohistochemical analysis.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Immunohistochemistry of breast carcinoma staining E Cadherin with ab40772 at 1μg/ml

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      OI-RD Scanning - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

      Produced using unpurified ab40772

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    • Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)
      Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (464)

    Publishing research using ab40772? Please let us know so that we can cite the reference in this datasheet.

    ab40772 has been referenced in 464 publications.

    • Wang D  et al. Loss of 4.1N in epithelial ovarian cancer results in EMT and matrix-detached cell death resistance. Protein Cell 12:107-127 (2021). PubMed: 32448967
    • Luo J  et al. miR-576-5p promotes epithelial-to-mesenchymal transition in colorectal cancer by targeting the Wnt5a-mediated Wnt/ß-catenin signaling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33300054
    • Chen Z  et al. NORAD regulates epithelial-mesenchymal transition of non-small cell lung cancer cells via miR-422a. Mol Med Rep 23:N/A (2021). PubMed: 33300080
    • Xiao S  et al. Polygalacin D suppresses esophageal squamous cell carcinoma growth and metastasis through regulating miR-142-5p/Nrf2 axis. Free Radic Biol Med 164:58-75 (2021). PubMed: 33307164
    • He W  et al. miR-182-5p contributes to radioresistance in nasopharyngeal carcinoma by regulating BNIP3 expression. Mol Med Rep 23:N/A (2021). PubMed: 33313953
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 28 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Tissue (Human Primary Bronchial Epithelial Cells in Algina)
    Specification
    Human Primary Bronchial Epithelial Cells in Algina
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 28 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (BeWo choriocarcinoma)
    Permeabilization
    Yes - 0.1 TritonX-100 in PBS
    Specification
    BeWo choriocarcinoma
    Blocking step
    BlockAid Reagent as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Shuhiba Mohammad

    Verified customer

    Submitted Aug 21 2021

    Western blot abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BeWo choriocarcinoma)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    20 µg
    Treatment
    25µM, 100µM, DMSO 48hrs (Forskolin treatment)
    Specification
    BeWo choriocarcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Shuhiba Mohammad

    Verified customer

    Submitted Jul 17 2021

    Western blot abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PDAC cell lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    PDAC cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    DR. Carlos Melo

    Verified customer

    Submitted Jun 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Head and Neck Cancer (Xenograft))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris Buffer pH9
    Permeabilization
    Yes - ready to use - unknown
    Specification
    Head and Neck Cancer (Xenograft)
    Blocking step
    Protein Block Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Ms. Loreen Weichert

    Verified customer

    Submitted Jun 14 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Dec 01 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Kupffer Cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Kupffer Cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 21 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (epithelial)
    Permeabilization
    No
    Specification
    epithelial
    Blocking step
    Vector Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (H2291 Lung Cancer cell line)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    H2291 Lung Cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 05 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6 Citrate
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 11 2018

    1-10 of 28 Abreviews or Q&A

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