Anti-E1B-AP5 antibody [EP2633Y] (ab68480)
Key features and details
- Rabbit monoclonal [EP2633Y] to E1B-AP5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-E1B-AP5 antibody [EP2633Y]
See all E1B-AP5 primary antibodies -
Description
Rabbit monoclonal [EP2633Y] to E1B-AP5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human E1B-AP5 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Wild-type HEK293T, Raji, and HeLa cell lysates. IHC-P: Human brain tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2633Y -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro. -
Sequence similarities
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
Domain
The RGG-box domain is methylated. -
Post-translational
modificationsMethylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11100 Human
- Omim: 605800 Human
- SwissProt: Q9BUJ2 Human
- Unigene: 155218 Human
- Unigene: 718642 Human
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Alternative names
- Adenovirus early region 1B associated protein 5 antibody
- Adenovirus early region 1B-associated protein 5 antibody
- E130317O14Rik antibody
see all
Images
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All lanes : Anti-E1B-AP5 antibody [EP2633Y] (ab68480) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : HNRNPUL1 knockout HEK293T cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 96 kDa
Observed band size: 96 kDaLanes 1-3: Merged signal (red and green). Green - ab68480 observed at 96 kDa. Red - loading control ab8245 observed at 36 kDa.
ab68480 Anti-E1B-AP5 antibody [EP2633Y] was shown to specifically react with E1B-AP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266142 (knockout cell lysate ab257993) was used. Wild-type and E1B-AP5 knockout samples were subjected to SDS-PAGE. ab68480 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab68480 at 1/250 dilution staining E1B-AP5 in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-E1B-AP5 antibody [EP2633Y] (ab68480) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit, HRP conjugated at 1/2000 dilution
Predicted band size: 96 kDa
Observed band size: 96 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab68480 has been referenced in 5 publications.
- Batra J et al. Non-canonical proline-tyrosine interactions with multiple host proteins regulate Ebola virus infection. EMBO J 40:e105658 (2021). PubMed: 34260076
- Xiao R et al. Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. Cell 178:107-121.e18 (2019). PubMed: 31251911
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). PubMed: 30247708
- Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . PubMed: 25299183
- Massimelli MJ et al. Interplay between polyadenylate-binding protein 1 and Kaposi's sarcoma-associated herpesvirus ORF57 in accumulation of polyadenylated nuclear RNA, a viral long noncoding RNA. J Virol 87:243-56 (2013). PubMed: 23077296