Anti-E2F5 antibody [220D1a] (ab59769)
Key features and details
- Mouse monoclonal [220D1a] to E2F5
- Suitable for: WB
- Isotype: IgG1
Overview
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Product name
Anti-E2F5 antibody [220D1a]
See all E2F5 primary antibodies -
Description
Mouse monoclonal [220D1a] to E2F5 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Immunogen
Recombinant fragment (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
220D1a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. -
Sequence similarities
Belongs to the E2F/DP family. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- E2F 5 antibody
- E2F transcription factor 5 antibody
- E2F transcription factor 5 p130 binding antibody
see all
Images
Datasheets and documents
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Datasheet download
References (6)
ab59769 has been referenced in 6 publications.
- Jiang G et al. hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis. Biomed Res Int 2022:6918396 (2022). PubMed: 35909488
- Xie Y et al. CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway. Cancer Cell Int 21:410 (2021). PubMed: 34348712
- Wang L et al. A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo. PLoS One 8:e85650 (2013). PubMed: 24392024
- Uboldi S et al. A systems biology approach to characterize the regulatory networks leading to trabectedin resistance in an in vitro model of myxoid liposarcoma. PLoS One 7:e35423 (2012). WB . PubMed: 22523595
- Jiang Y et al. A potential oncogenic role of the commonly observed E2F5 overexpression in hepatocellular carcinoma. World J Gastroenterol 17:470-7 (2011). WB, IHC-P ; Human . PubMed: 21274376
- Wan Z et al. Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors. J Clin Invest 120:3530-44 (2010). WB ; Human . PubMed: 20890043