Anti-E4F1 antibody (ab70615)
Key features and details
- Rabbit polyclonal to E4F1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E4F1 antibody
See all E4F1 primary antibodies -
Description
Rabbit polyclonal to E4F1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human E4F1. Corresponding to a region between residue 275 and 325 of human E4F1
Database link: Q66K89 -
Positive control
- HeLa, 293T, and MCF7 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70615 was affinity purified using an epitope specific to E4F1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa).
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IP |
Use at 2 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa). |
IP
Use at 2 µg/mg of lysate. |
Target
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Relevance
E4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 1877 Human
- Omim: 603022 Human
- SwissProt: Q66K89 Human
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Alternative names
- E4F antibody
- E4F transcription factor 1 antibody
- E4F transcription factor 1, isoform CRA_b antibody
see all
Images
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All lanes : Anti-E4F1 antibody (ab70615) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 84 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of Human E4F1 by Western Blot of Immunprecipitate. ab70615 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70615 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70615 has been referenced in 1 publication.
- Gui Z et al. Poly(ADP-ribose) polymerase-1 promotes expression of miR-155 by the up-regulation of methyl-CpG binding domain protein 2 in TK6 cells exposed to hydroquinone. Toxicol In Vitro 55:51-57 (2019). PubMed: 30448557