Anti-EBNA 3A antibody (ab16126)
Key features and details
- Sheep polyclonal to EBNA 3A
- Suitable for: ICC, Dot blot, ELISA, IHC-Fr, WB
- Isotype: IgG
Overview
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Product name
Anti-EBNA 3A antibody -
Description
Sheep polyclonal to EBNA 3A -
Host species
Sheep -
Specificity
This antibody is specific for the Epstein Barr Virus Nuclear Antigen 3A. -
Tested applications
Suitable for: ICC, Dot blot, ELISA, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Epstein-Barr virus -
Immunogen
Recombinant full length protein. Recombinant EBNA-3A protein
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Positive control
- WB: EBV infected tissues and cells
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General notes
Sheep were immunized with recombinant EBNA-3A protein
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified by ammonium sulfate precipitation. This antibody is provided as a 0.2µm sterile filtered solution. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 0.25 - 1 µg/ml.
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Notes |
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ICC
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use a concentration of 0.25 - 1 µg/ml. |
Target
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Relevance
EBNA3A is a latent viral nuclear protein expressed in Epstein Barr Virus (EBV) transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA3A functions as a transcriptional regulator though the target genes are currently unknown. -
Database links
- SwissProt: Q69138 Epstein-Barr virus
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Alternative names
- EBNA 3A antibody
- EBV antibody
- Epstein Barr virus antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab16126 has been referenced in 11 publications.
- Gain C et al. Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway. PLoS Pathog 16:e1008105 (2020). PubMed: 32092124
- Barzyk GA & Sheriff V EBV Positivity and Programmed Death-ligand 1 Expression in Diffuse Large B-cell Lymphoma: A Systematic Review. Anticancer Res 40:5951-5968 (2020). PubMed: 33109533
- Paschos K et al. Requirement for PRC1 subunit BMI1 in host gene activation by Epstein-Barr virus protein EBNA3C. Nucleic Acids Res N/A:N/A (2019). PubMed: 30649516
- Gillman ACT et al. Epstein-Barr Virus Nuclear Antigen 3C Inhibits Expression of COBLL1 and the ADAM28-ADAMDEC1 Locus via Interaction with the Histone Lysine Demethylase KDM2B. J Virol 92:N/A (2018). PubMed: 30135119
- Szymula A et al. Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome. PLoS Pathog 14:e1006890 (2018). PubMed: 29462212