Anti-ECH1 antibody (ab153720)
Key features and details
- Rabbit polyclonal to ECH1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ECH1 antibody
See all ECH1 primary antibodies -
Description
Rabbit polyclonal to ECH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 97-328 of Human ECH1 (Uniprot ID: Q13011).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family. -
Cellular localization
Mitochondrion. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 1891 Human
- Entrez Gene: 51798 Mouse
- Entrez Gene: 733679 Pig
- Omim: 600696 Human
- SwissProt: Q13011 Human
- SwissProt: O35459 Mouse
- Unigene: 196176 Human
- Unigene: 291776 Mouse
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Alternative names
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
- Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
see all
Images
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All lanes : Anti-ECH1 antibody (ab153720) at 1/2000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : ECH1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa -
Anti-ECH1 antibody (ab153720) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 36 kDa
10% SDS PAGE -
Anti-ECH1 antibody (ab153720) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 36 kDa
10% SDS PAGE -
Confocal immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECH1 with ab153720 at 1/500 dilution (green). Alpha-tubulin filaments labeled in red.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab153720 has been referenced in 3 publications.
- Fiorentino TV et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378
- Mao X et al. Enoyl coenzyme A hydratase 1 combats obesity and related metabolic disorders by promoting adipose tissue browning. Am J Physiol Endocrinol Metab 318:E318-E329 (2020). PubMed: 31961704
- Strickaert A et al. Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer. Thyroid 29:845-857 (2019). PubMed: 30990120