Overview

  • Product name
  • Description
    Rabbit polyclonal to ECHS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 55-290 of Human ECHS1 (Uniprot ID: P30084).

  • Positive control
    • NT2D1, PC3, U-87 MG, SK N SH, Neuro 2A, GL261 and C8D30 whole cell lysates; Human NCI-N87 xenograft tissue; HeLa cells.

Applications

Our Abpromise guarantee covers the use of ab153732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway.
  • Cellular localization
    Mitochondrion matrix.
  • Database links
  • Alternative names
    • C80529 antibody
    • EC 4.2.1.17 antibody
    • ECHS 1 antibody
    • Enoyl CoA hydratase 1 antibody
    • Enoyl CoA hydratase mitochondrial antibody
    • Enoyl CoA hydratase short chain 1 mitochondrial antibody
    • Enoyl Coenzyme A hydratase short chain 1 antibody
    • Enoyl Coenzyme A hydratase short chain 1 mitochondrial antibody
    • Mitochondrial short chain enoyl coenzyme A hydratase 1 antibody
    • OTTHUMP00000020811 antibody
    • SCEH antibody
    • Short chain enoyl CoA hydratase antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECHS1 with ab153732 at 1/200 dilution. Right cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue, labeling ECHS1 with ab153732 at 1/250 dilution.
  • All lanes : Anti-ECHS1 antibody (ab153732) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 31 kDa



    12% SDS PAGE
  • All lanes : Anti-ECHS1 antibody (ab153732) at 1/3000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC3 whole cell lysate
    Lane 3 : U-87 MG whole cell lysate
    Lane 4 : SK N SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 31 kDa



    12% SDS PAGE

References

This product has been referenced in:
  • Yamada K  et al. Clinical, biochemical and metabolic characterisation of a mild form of human short-chain enoyl-CoA hydratase deficiency: significance of increased N-acetyl-S-(2-carboxypropyl)cysteine excretion. J Med Genet 52:691-8 (2015). Read more (PubMed: 26251176) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153732.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up