Anti-EDG8 antibody (ab92994)
Key features and details
- Rabbit polyclonal to EDG8
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EDG8 antibody
See all EDG8 primary antibodies -
Description
Rabbit polyclonal to EDG8 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following lysates: Brain: corpus callosum (Human) Whole Cell Lysate; Brain (Human) Tissue Lysate; Spleen (Human) Tissue Lysate; Lung (Human) Tissue Lysate; U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate; MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 42 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells. -
Tissue specificity
Widely expressed in the brain, most prominently in the corpus callosum, which is predominantly white matter. Detected in spleen, peripheral blood leukocytes, placenta, lung, aorta and fetal spleen. Low-level signal detected in many tissue extracts. Overexpressed in leukemic large granular lymphocytes. Isoform 1 is predominantly expressed in peripheral tissues. Isoform 2 is expressed in brain, spleen and peripheral blood leukocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Developmental stage
At 24 weeks of gestation, fragments of radial glial fibers are positive within the cortical plate and subplate of allocortical areas. These positive fragments often appear enlarged as varicosities and some of them terminate at blood vessels. Between 28 and 30 weeks of gestation, all iso- and allocortical areas contain immunolabelled radial glial fibers revealing curvature next to sulci. After 32 weeks of gestation, radial glial fibers gradually disappear; instead positive transitional stages between radial glia and astrocytes were found. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 53637 Human
- Entrez Gene: 94226 Mouse
- Entrez Gene: 60399 Rat
- Omim: 605146 Human
- SwissProt: Q9H228 Human
- SwissProt: Q91X56 Mouse
- SwissProt: Q9JKM5 Rat
- Unigene: 501561 Human
see all -
Alternative names
- EDG 8 antibody
- EDG8 S1P receptor antibody
- Endothelial differentiation G protein coupled receptor 8 antibody
see all
Images
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All lanes : Anti-EDG8 antibody (ab92994) at 1 µg/ml
Lane 1 : Human brain corpus callosum tissue lysate (alzheimer) - total protein (ab30027)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab92994 has been referenced in 3 publications.
- Gläser A et al. Identification of Brain-Specific Treatment Effects in NPC1 Disease by Focusing on Cellular and Molecular Changes of Sphingosine-1-Phosphate Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32599915
- Palangi A et al. Differential expression of S1P receptor subtypes in human bladder transitional cell carcinoma. Clin Transl Oncol N/A:N/A (2019). PubMed: 30712233
- Arana L et al. Ceramide 1-phosphate induces macrophage chemoattractant protein-1 release: involvement in ceramide 1-phosphate-stimulated cell migration. Am J Physiol Endocrinol Metab 304:E1213-26 (2013). PubMed: 23548612