Anti-MICU2 antibody (ab101465)
Key features and details
- Rabbit polyclonal to MICU2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MICU2 antibody -
Description
Rabbit polyclonal to MICU2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human MICU2 aa 95-238. (AAH31089)
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Positive control
- U87-MG whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Sequence similarities
Contains 4 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 221154 Human
- Entrez Gene: 68514 Mouse
- Omim: 610632 Human
- SwissProt: Q8IYU8 Human
- SwissProt: Q8CD10 Mouse
- Unigene: 412103 Human
- Unigene: 26834 Mouse
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Alternative names
- 1110008L20Rik antibody
- EF hand domain containing family member A1 antibody
- EF hand domain family A1 antibody
see all
Images
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Anti-MICU2 antibody (ab101465) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS-PAGE -
Immunohistochemical analysis of FFPE mouse kidney tissue, labeling MICU2 with ab101465 (diluted 1/500).
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Immunohistochemical analysis of FFPE human hepatoma tissue, labeling MICU2 with ab101465 (diluted 1/500).
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Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF-7 cell line, labeling MICU2 with ab101465 (diluted 1/200). DNA stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab101465 has been referenced in 27 publications.
- Singh R et al. Uncontrolled mitochondrial calcium uptake underlies the pathogenesis of neurodegeneration in MICU1-deficient mice and patients. Sci Adv 8:eabj4716 (2022). PubMed: 35302860
- Chiu HY et al. Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma. Cell Death Dis 13:419 (2022). PubMed: 35490194
- Devine MJ et al. Mitochondrial Ca2+ uniporter haploinsufficiency enhances long-term potentiation at hippocampal mossy fibre synapses. J Cell Sci 135:N/A (2022). PubMed: 36274588
- Vishnu N et al. Mitochondrial clearance of calcium facilitated by MICU2 controls insulin secretion. Mol Metab 51:101239 (2021). PubMed: 33932586
- Wu R et al. MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells. Biomed Res Int 2021:6650791 (2021). PubMed: 34189138