Overview

  • Product name

  • Description

    Rabbit polyclonal to EHHADH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of Human EHHADH (NP_001957.2).

  • Positive control

    • WB: mouse liver and kidney tissue lysates IHC-P: human hepatocarcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab93172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 79 kDa.
IHC-P 1/50 - 1/100.

Target

  • Tissue specificity

    Liver and kidney. Lower amounts seen in the brain.
  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • Post-translational
    modifications

    Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
  • Cellular localization

    Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 hydroxyacyl CoA dehydrogenase antibody
    • 3,2 trans enoyl CoA isomerase antibody
    • 3-hydroxyacyl-CoA dehydrogenase antibody
    • ECHD antibody
    • ECHP_HUMAN antibody
    • EHHADH antibody
    • Enoyl Coenzyme A antibody
    • Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • L 3 hydroxyacyl CoA dehydrogenase antibody
    • L bifunctional protein antibody
    • L bifunctional protein, peroxisomal antibody
    • L PBE antibody
    • LBFP antibody
    • LBP antibody
    • MGC120586 antibody
    • MS730 antibody
    • PBE antibody
    • PBFE antibody
    • Peroxisomal bifunctional enzyme antibody
    • Peroxisomal enoyl CoA hydratase antibody
    see all

Images

  • All lanes : Anti-EHHADH antibody (ab93172) at 1/100 dilution

    Lane 1 : mouse liver tissue lysates
    Lane 2 : mouse kidney tissue lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 79 kDa

  • ab93172, at a 1/50 dilution, staining EHHADH in formalin-fixed and paraffin-embedded Human hepatocarcinoma peroxidase-conjugated to a secondary antibody, followed by DAB staining.

References

This product has been referenced in:

  • Tsoli M  et al. Depletion of white adipose tissue in cancer cachexia syndrome is associated with inflammatory signaling and disrupted circadian regulation. PLoS One 9:e92966 (2014). WB ; Mouse . Read more (PubMed: 24667661) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry and I am sorry to hear that you are having some trouble with this antibody.

We sell the mouse liver whole cell lysate as ab29301:

https://www.abcam.com/Liver-Mouse-Whole-Cell-Lysate-normal-tissue-ab29301.html

I would recommend purchasing the positive control. If you're still not seeing bands with the antibody using the positive control, let me know and we can replace or refund the antibody if it was purchased within the last 6 months.

I hope this information helps. Please contact us with any other questions.

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