Overview

  • Product name
    Anti-ELF2 antibody [224C4a]
    See all ELF2 primary antibodies
  • Description
    Mouse monoclonal [224C4a] to ELF2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of Human ELF2 from an internal sequence.

  • Positive control
    • Recombinant ELF2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab70597 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    224C4a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 64 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Relevance
    The ELF2 gene encodes a protein that physically interacts with AML1 and mediates opposing effects on AML1 mediated transcription of the B cell specific blk gene.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • E74 like factor 2 antibody
    • E74 like factor 2 ets domain transcription factor antibody
    • ELF 2 antibody
    • Ets domain transcription factor antibody
    • Ets related factor antibody
    • ETS related transcription factor Elf 2 antibody
    • ETS related transcription factor Elf2 antibody
    • EU 32 antibody
    • EU32 antibody
    • HGNC:3317 antibody
    • NERF 1A antibody
    • NERF 1a b antibody
    • NERF 1B antibody
    • NERF 2 antibody
    • NERF antibody
    • NERF1A antibody
    • NERF1a b antibody
    • NERF1B antibody
    • NERF2 antibody
    • New ETS related factor antibody
    see all

Images

  • Anti-ELF2 antibody [224C4a] (ab70597) + immunising recombinant protein

    Predicted band size: 64 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References

ab70597 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

To our knowledge, this product has not been tested in Zebrafish. Alignment of the immunogen sequence to D.rerio E74-like factor-2 (Sequence ID:NP954689.1) shows an identity score of 79% with positives 85%. The latter part of the protein has great homology. While we have not yet mapped the epitope, if this is within the later 2/3, we would expect reaction.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up