Anti-ELOVL6/LCE antibody (ab69857)
Key features and details
- Rabbit polyclonal to ELOVL6/LCE
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELOVL6/LCE antibody -
Description
Rabbit polyclonal to ELOVL6/LCE -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
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Positive control
- WB: Human brain tissue lysate. IHC-P: Human brain tissue. ICC/IF: Human brain cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with ELOVL6/LCE peptide (ab114079).
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with ELOVL6/LCE peptide (ab114079). |
Target
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Function
Condensing enzyme that catalyzes the synthesis of saturated and monounsaturated fatty acids. Highest activity toward C16:0 acyl-CoAs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ELO family. -
Post-translational
modificationsN-Glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 79071 Human
- Entrez Gene: 170439 Mouse
- Entrez Gene: 171402 Rat
- Omim: 611546 Human
- SwissProt: Q9H5J4 Human
- SwissProt: Q920L5 Mouse
- SwissProt: Q920L6 Rat
- Unigene: 412939 Human
see all -
Alternative names
- 3-keto acyl-CoA synthase ELOVL6 antibody
- Elongation of very long chain fatty acids protein 6 antibody
- ELOV6_HUMAN antibody
see all
Images
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Lane 1 : Anti-ELOVL6/LCE antibody (ab69857) at 1 µg/ml
Lane 2 : Anti-ELOVL6/LCE antibody (ab69857) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunohistochemistry of ELOVL6/LCE in human brain with ab69857 at 5 µg/ml.
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Immunofluorescence of ELOVL6/LCE in Human Brain cells using ab69857 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab69857 has been referenced in 11 publications.
- Jiang P et al. Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells. Arch Anim Breed 65:357-370 (2022). PubMed: 36304442
- Castriota F et al. Chronic arsenic exposure impairs adaptive thermogenesis in male C57BL/6J mice. Am J Physiol Endocrinol Metab 318:E667-E677 (2020). PubMed: 32045263
- Romero-Córdoba SL et al. Mexican Ganoderma Lucidum Extracts Decrease Lipogenesis Modulating Transcriptional Metabolic Networks and Gut Microbiota in C57BL/6 Mice Fed with a High-Cholesterol Diet. Nutrients 13:N/A (2020). PubMed: 33374283
- Mika A et al. Exercise and Conjugated Linoleic Acid Supplementation Induce Changes in the Composition of Liver Fatty Acids. Front Physiol 10:602 (2019). PubMed: 31156468
- Li H et al. Decreased expression levels of ELOVL6 indicate poor prognosis in hepatocellular carcinoma. Oncol Lett 18:6214-6220 (2019). PubMed: 31788097