FITC Anti-ENPP3/B10 antibody [NP4D6] (ab239252)
Key features and details
- FITC Mouse monoclonal [NP4D6] to ENPP3/B10
- Suitable for: Flow Cyt, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-ENPP3/B10 antibody [NP4D6]
See all ENPP3/B10 primary antibodies -
Description
FITC Mouse monoclonal [NP4D6] to ENPP3/B10 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cyt, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human ENPP3/B10. (HEK-293 cells transfected with human CD203c).
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Positive control
- Flow Cyt: Human peripheral blood. Flow Cyt (Intra): Human peripheral blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
NP4D6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 4µl for 106 cells.
(or 100 µl of whole blood). |
|
Flow Cyt (Intra) |
Use 4µl for 106 cells.
(or 100 μl of whole blood). |
Notes |
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Flow Cyt
Use 4µl for 106 cells. (or 100 µl of whole blood). |
Flow Cyt (Intra)
Use 4µl for 106 cells. (or 100 μl of whole blood). |
Target
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Function
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. -
Tissue specificity
Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains. -
Post-translational
modificationsN-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. -
Cellular localization
Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 5167 Human
- Entrez Gene: 5169 Human
- Omim: 602182 Human
- SwissProt: O14638 Human
- SwissProt: P22413 Human
- Unigene: 486489 Human
- Unigene: 527295 Human
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Alternative names
- Alkaline phosphodiesterase I antibody
- ARHR2 antibody
- B10 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab239252 has not yet been referenced specifically in any publications.