Overview

  • Product name

  • Description

    Mouse monoclonal to EPCR/CD201
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-239 of Human EPCR/CD201

  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
  • Tissue specificity

    Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
  • Post-translational
    modifications

    N-glycosylated.
    A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activated protein C receptor antibody
    • APC receptor antibody
    • APCR antibody
    • bA42O4.2 antibody
    • CCCA antibody
    • CCD41 antibody
    • CD201 antibody
    • CD201 antigen antibody
    • cell cycle, centrosome-associated protein antibody
    • Cell cycle, centrosome-associated protein antibody
    • centrocyclin antibody
    • Endothelial cell protein C receptor antibody
    • Endothelial protein C receptor antibody
    • EPCR antibody
    • EPCR_HUMAN antibody
    • MGC23024 antibody
    • PROCR antibody
    • Protein C receptor, endothelial antibody
    see all

Images

  • Overlay histogram showing A549 cells stained with ab56689 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56689, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    This image was generated using the ascites version of the product.

  • EPCR/CD201 antibody (ab56689) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.

    This image was generated using the ascites version of the product.

  • Western blot against tagged recombinant protein immunogen using ab56689 EPCR/CD201 antibody at 1ug/ml. Predicted band size of immunogen is 52 kDa

    This image was generated using the ascites version of the product.

  • Immunofluorescence analysis of HeLa cells, staining EPCR/CD201 with ab56689 at 10 μ/ml.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Wei Y  et al. Effect of cigarette smoke extract and nicotine on the expression of thrombomodulin and endothelial protein C receptor in cultured human umbilical vein endothelial cells. Mol Med Rep 17:1724-1730 (2018). Read more (PubMed: 29257196) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for getting in touch.
The mouse monoclonal anti-EPCR/CD201 antibody (ab56689) is from clone M2.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up