Immunocytochemistry/ Immunofluorescence abreview for Anti-ERK1 + ERK2 antibody [EPR17526]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were left untreated, or treated with either PBDu (1uM 30min) or U0126 (10uM 1 h), paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Following PDBu treatment, this antibody appears to become highly enriched within nuclei versus a more diffuse and equally distributed pattern (cytoplasm + nucleus) in untreated cells. Following U0126 treatment, this antibody does not appear to change localization and appears similar to the untreated condition.

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 26 2017

Sign up