Anti-ETHE1 antibody (ab251791)
Key features and details
- Rabbit polyclonal to ETHE1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ETHE1 antibody
See all ETHE1 primary antibodies -
Description
Rabbit polyclonal to ETHE1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human ETHE1 aa 150-250.
Database link: O95571 -
Positive control
- IHC-P: Human colon, kidney and liver tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 28 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 28 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probably plays an important role in metabolic homeostasis in mitochondria. May function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. Suppresses p53-induced apoptosis by preventing nuclear localization of RELA. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in ETHE1 are a cause of ethylmalonic encephalopathy (EE) [MIM:602473]. EE is an autosomal recessive disorder characterized by neurodevelopmental delay and regression, recurrent petechiae, acrocyanosis, diarrhea, leading to death in the first decade of life. It is also associated with persistent lactic acidemia and ethylmalonic and methylsuccinic aciduria. -
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. -
Cellular localization
Cytoplasm. Nucleus. Mitochondrion matrix. According to PubMed:12398897, it is cytoplasmic and nuclear. According to PubMed:14732903, it is found in the mitochondrial matrix. - Information by UniProt
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Database links
- Entrez Gene: 23474 Human
- Entrez Gene: 66071 Mouse
- Entrez Gene: 292710 Rat
- Omim: 608451 Human
- SwissProt: O95571 Human
- SwissProt: Q9DCM0 Mouse
- Unigene: 7486 Human
- Unigene: 29553 Mouse
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Alternative names
- Ethe1 antibody
- ETHE1 protein, mitochondrial precursor antibody
- ETHE1_HUMAN antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ETHE1 (green) using ab251791 at 4 µg/ml in ICC/IF.
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All lanes : Anti-ETHE1 antibody (ab251791) at 0.4 µg/ml
Lane 1 : RT-4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : Human U-251-MG sp whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 28 kDa -
Formalin-fixed, paraffin-embedded human colon tissue stained for ETHE1 with ab251791 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for ETHE1 with ab251791 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for ETHE1 with ab251791 at a 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-ETHE1 antibody (ab251791) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II whole cell lysate
Predicted band size: 28 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab251791 has not yet been referenced specifically in any publications.