• Product name
    Anti-ETS1 (phospho T38) antibody
    See all ETS1 primary antibodies
  • Description
    Rabbit polyclonal to ETS1 (phospho T38)
  • Host species
  • Specificity
    Detects endogenous levels of ETS1 only when phosphorylated at threonine 38.
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide from around the phosphorylation site of threonine 38 (LLTPPS) of human ETS1

  • Positive control
    • Human breast carcinoma tissue. IF/ICC: HepG2 cell line.



Our Abpromise guarantee covers the use of ab59179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).


  • Function
    Transcription factor.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Post-translational
    Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • c-ets-1 antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS proto-oncogene 1, transcription factor antibody
    • ETS1 antibody
    • ETS1 oncogene antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • Oncogene ETS1 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all


  • All lanes : Anti-ETS1 (phospho T38) antibody (ab59179) at 1/1000 dilution

    Lane 1 : Human A2058 whole cell lysate
    Lane 2 : Human A375 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    All lanes : HRP-conjugated Hourse anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 110 kDa (possible non-specific binding), 180 kDa (possible non-specific binding), 70 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)

    Exposure time: 5 minutes

    See Abreview

  • ICC/IF image of ab59179 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59179 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.


This product has been referenced in:
  • Sinh ND  et al. Ets1 and ESE1 reciprocally regulate expression of ZEB1/ZEB2, dependent on ERK1/2 activity, in breast cancer cells. Cancer Sci 108:952-960 (2017). Read more (PubMed: 28247944) »
  • Chen J  et al. VEGF amplifies transcription through ETS1 acetylation to enable angiogenesis. Nat Commun 8:383 (2017). Read more (PubMed: 28851877) »

See all 4 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE)
Human Cell lysate - whole cell (A2058 and A375)
A2058 and A375
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 16 2013

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab59179 has never been tested in Western blot. All tested applications covered by the 6 month guarantee are specified on our datasheets, and these are updated as ...

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Thank you for your call today. Please see below for information about your testing discount code, and let me know if you have any questions.

Expiration date: September 24, 2012

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