• Product name
  • Description
    Rabbit polyclonal to EYA2
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 100 and 150 of human EYA2 (NP_005235.3)

  • Positive control
    • 293T cells lysate



Our Abpromise guarantee covers the use of ab95875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
  • Tissue specificity
    Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
  • Sequence similarities
    Belongs to the HAD-like hydrolase superfamily. EYA family.
  • Developmental stage
    At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAB 1 antibody
    • EAB1 antibody
    • EYA 2 antibody
    • EYA transcriptional coactivator and phosphatase 2 antibody
    • EYA2 antibody
    • EYA2_HUMAN antibody
    • eyes absent 2 homolog (Drosophila) antibody
    • Eyes absent homolog 2 (Drosophila) antibody
    • Eyes absent homolog 2 antibody
    • Translation of this uORF probably lowers the translation efficiency of EYA2 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA2 with ab95875 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EYA2 antibody (ab95875) at 0.2 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa

    Exposure time: 30 seconds
  • Detection of human Eya2 by Western Blot of Immunoprecipitate. ab95875 at 0.1µg/ml staining Eya2 in 293T cell lysate immunoprecipitated using ab95875 at 3µg/mg (1mg/IP; 20% of IP loaded/lane) Detection: Chemiluminescence with exposure time of 10 seconds..


This product has been referenced in:
  • Li Z  et al. EYA2 promotes lung cancer cell proliferation by downregulating the expression of PTEN. Oncotarget 8:110837-110848 (2017). Read more (PubMed: 29340020) »

See 1 Publication for this product

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