Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)
Key features and details
- Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
- Isotype: IgG1
Overview
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Product name
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies -
Description
Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
Predicted to work with: Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
Sequence:HTGFLpTEpYVAT
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Epitope
The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2). -
Positive control
- Rat brain extract, Drosophila wing imaginal disc.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
MAPK-YT -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
1/200.
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WB | (8) |
1/10000. Predicted molecular weight: 44 kDa.
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Notes |
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ICC/IF
1/200. |
WB
1/10000. Predicted molecular weight: 44 kDa. |
Images
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Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody
All lanes : Extract of Rat1 cells activated with 100µM
Vanadate+200µM H2O2
Secondary
All lanes : Goat Anti-Mouse IgG, AlkPhos
Conjugate
Predicted band size: 44 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted? -
In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
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ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (120)
ab50011 has been referenced in 120 publications.
- Wu X et al. Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma. Photoacoustics 26:100355 (2022). PubMed: 35479192
- Liu C et al. Disrupted tenogenesis in masseter as a potential cause of micrognathia. Int J Oral Sci 14:50 (2022). PubMed: 36257937
- Li N et al. Altered BMP-Smad4 signaling causes complete cleft palate by disturbing osteogenesis in palatal mesenchyme. J Mol Histol 52:45-61 (2021). PubMed: 33159638
- Tao J et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
- Zhang C et al. Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury. Mediators Inflamm 2021:6684120 (2021). PubMed: 33776576