Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Overview

  • Product name

    Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal
  • Description

    Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
  • Host species

    Mouse
  • Specificity

    Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human Estrogen Receptor beta 1 (C terminal).
    Database link: Q92731

  • Positive control

    • MCF-7 cells,ovarian, breast or prostate carcinoma tissues, bladder, gastric or salivary carcinoma

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab187291 was purified from Bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    ERb455
  • Isotype

    IgG2a

Applications

Our Abpromise guarantee covers the use of ab187291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Flow Cyt Use 0.5-1µg for 106 cells.

Target

  • Relevance

    This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • ER-BETA antibody
    • Erb antibody
    • ESR-BETA antibody
    • ESRB antibody
    • ESTRB antibody
    • Estrogen nuclear receptor beta variant a antibody
    • Estrogen nuclear receptor beta variant b antibody
    • Estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded ovarian cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Gastric Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Bladder Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Flow cytometry analysis of  BT474 cells labelling ER beta with ab187291 at 0.5 ug/ml .Black-cells alone,Grey-isotype control,Green-AF4888-labeled ER beta 1.

  • Flow cytometry analysis of MCF-7 cells labelling ER beta with ab187291 at 0.5 ug/ml .Black-cells alone,Green-isotype control,Red-PE labeled ER-beta 1.

References

ab187291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 05 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 31 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (vagina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
vagina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (oviduct)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
oviduct
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 15 2015

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