• Product name
    Anti-Estrogen Related Receptor gamma antibody
    See all Estrogen Related Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Related Receptor gamma
  • Host species
  • Specificity
    This antibody detects human Estrogen Related Receptor gamma from transfected cells and shows no cross reactivity to ERR1.
  • Tested applications
    Suitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 401-413 of Human Estrogen Related Receptor gamma. This sequence is completely conserved between human, mouse and rat. (Peptide available as ab39779.)

  • Positive control
    • Recombinant Human Estrogen Related Receptor gamma protein (ab114455) can be used as a positive control in WB. COS7 or HEK293 transfected cells. Human brain, kidney or pancreas.



Our Abpromise guarantee covers the use of ab16366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human Estrogen Related Receptor gamma peptide (ab39779). Representing Estrogen Related Receptor gamma from COS7 transfected cells.


  • Function
    Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
  • Tissue specificity
    Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stage
    Expressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
  • Post-translational
    Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity.
    Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781L1617 antibody
    • ERR 3 antibody
    • ERR G2 antibody
    • ERR gamma 2 antibody
    • ERR gamma-2 antibody
    • ERR3 antibody
    • ERR3_HUMAN antibody
    • ERRG 2 antibody
    • ERRG2 antibody
    • ERRgamma antibody
    • ESRRG antibody
    • Estrogen receptor related protein 3 antibody
    • Estrogen receptor-related protein 3 antibody
    • Estrogen-related receptor gamma antibody
    • FLJ16023 antibody
    • KIAA0832 antibody
    • NR3B3 antibody
    • Nuclear receptor subfamily 3 group B member 3 antibody
    see all


  • Lanes 1-2 : Anti-Estrogen Related Receptor gamma antibody (ab16366) at 1/500 dilution
    Lane 3 : Anti-Estrogen Related Receptor gamma antibody (ab16366) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Mouse kidney cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 51 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?


ab16366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your enquiry. ERR3 (ab16366) is epitope affinity purified, but it appears there are a few non-specific bands around the 54 kDa range, which is why this product is recommended for cells overexpressing the protein. ERR3 has been used with transfected COS-7 and HEK293 human cells. In these transfected experiments, a single band at ~54 kDa is observed. Suitable controls are human brain, kidney or pancreas. The original reference for this product can be found at: Molecular Endocrinology 14(3):382-392, 2000. Conditions for blocking are very generic, 1-1 1/2 hours at room temperature in 5% milk. Please let me know if these suggestions do not help.

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