PE Anti-F4/80 antibody [CI:A3-1] (ab105156)
Key features and details
- PE Rat monoclonal [CI:A3-1] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
PE Anti-F4/80 antibody [CI:A3-1]
See all F4/80 primary antibodies -
Description
PE Rat monoclonal [CI:A3-1] to F4/80 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab105156 recognises the murine F4/80 antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse F4/80. Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.
-
Positive control
- Mouse J774 cells
-
General notes
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 5% Sucrose, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Primary antibody notes
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. -
Clonality
Monoclonal -
Clone number
CI:A3-1 -
Myeloma
NS1 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
in 100 µl. ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
---|
Flow Cyt
Use 10µl for 106 cells. in 100 µl. ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
-
Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab105156 has been referenced in 7 publications.
- Babazadeh S et al. Macrophage polarization by MSC-derived CXCL12 determines tumor growth. Cell Mol Biol Lett 26:30 (2021). PubMed: 34174813
- Liu X et al. Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization. Cell Death Dis 11:1016 (2020). PubMed: 33247124
- Shimizu Y et al. A simple culture method for liver and intestinal tissue-resident macrophages from neonatal mice. In Vitro Cell Dev Biol Anim 55:436-444 (2019). PubMed: 31119642
- Li H et al. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nat Commun 9:272 (2018). PubMed: 29348470
- Huang Z et al. The FGF21-CCL11 Axis Mediates Beiging of White Adipose Tissues by Coupling Sympathetic Nervous System to Type 2 Immunity. Cell Metab 26:493-508.e4 (2017). PubMed: 28844880
- Rokade S et al. Surfactant protein D regulates murine testicular immune milieu and sperm functions. Am J Reprod Immunol 77:N/A (2017). PubMed: 28054406
- Neng L et al. Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. Nat Protoc 8:709-20 (2013). PubMed: 23493068